Identification and Expression Characterization Analysis of the NRT2 Gene Family in Pepper (Capsicum annuum)
WU Yuan1,2,5, SU Shi-Xian1, WANG Tao1,2, LONG Cha1, WEI Jin-Jiang1,2, JIANG Su-Yan1, XING Dan3, YUAN Yuan-Guo4, LI Wei1,2,5,*
1 College of Agriculture, Guizhou University, Guiyang 550025, China; 2 Industry Technology Institute of Pepper, Guizhou University, Guiyang 550025, China; 3 Research Institute of Pepper, Guizhou Province, Zunyi 563000, China; 4 Guizhou Institute of Horticulture, Guiyang 550006, China; 5 Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions of Guizhou Province, Guiyang 550025, China
Abstract：Pepper (Capsicum annuum) is an important horticultural crop. NRT2 (nitrate transporter 2) gene families play a crucial role in nitrate absorption and transport. In order to explore the role of NRT2 gene in pepper growth and development, 8 pepper NRT2 genes were identified in this study using published pepper genome data and bioinformatics analysis methods. CaNRT2s family members were distributed on 4 chromosomes. There were some differences in protein structure and physical and chemical properties. According to phylogenetic analysis, CaNRT2s members were divided into 4 subfamilies, which were close to tomato (Lycopersicon esculentum) and potato (Solanum tuberosum) in evolutionary relationship. All members had MFS-1 conservative domain. Promoters contain a large number of hormone responsive elements, stress responsive elements and plant growth and development related elements. The expression of CaNRT2s were different in the developmental stage and tissue. CaNRT2.4 and CaNRT2.5 were highly expressed in roots, and CaNRT2.2 and CaNRT2.3 were highly expressed in multiple tissues and fruits during color transformation. The study found that the expressions of CaNRT2.1/2.2/2.3/2.8 were very low under various abiotic stresses and hormone treatment. The expressions of CaNRT2.4/2.5/2.6/2.7 were down regulated in leaves and up regulated in roots. CaNRT2.4/2.5/2.6/2.7 were up-regulated in leaves and roots after nitrogen deficiency treatment. The results were deduced that these 4 genes might be the key genes regulating nitrate transport in pepper. The research results provide theoretical basis for the cloning and functional study of NRT2 gene of NO3- absorption and transport in pepper.
吴媛, 苏世贤, 汪淘, 龙茶, 韦金江, 江苏燕, 邢丹, 袁远国, 李伟. 辣椒NRT2基因家族鉴定及表达特征分析[J]. 农业生物技术学报, 2023, 31(11): 2239-2253.
WU Yuan, SU Shi-Xian, WANG Tao, LONG Cha, WEI Jin-Jiang, JIANG Su-Yan, XING Dan, YUAN Yuan-Guo, LI Wei. Identification and Expression Characterization Analysis of the NRT2 Gene Family in Pepper (Capsicum annuum). 农业生物技术学报, 2023, 31(11): 2239-2253.
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