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2025年4月4日 星期五
农业生物技术学报  2023, Vol. 31 Issue (11): 2254-2262    DOI: 10.3969/j.issn.1674-7968.2023.11.004
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
甘蔗ScGAI基因两个启动子的组织特异性表达分析
柴哲, 方金兰, 黄润, 黄翠霖, 姚伟, 张木清*
广西大学 广西甘蔗生物学重点实验室/农学院,南宁 530005
Tissue specific Expressions Analysis of Two Promoters of Sugarcane (Saccharum officinarum) ScGAI Gene
CHAI Zhe, FANG Jin-Lan, HUANG Run, HUANG Cui-Lin, YAO Wei, ZHANG Mu-Qing*
Guangxi Key Laboratory of sugarcane biology/Agricultural College, Guangxi University, Nanning 530005, China
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摘要 甘蔗(Saccharum officinarum)是异源多倍体作物,其复杂的遗传背景导致甘蔗基因的调控机制更加复杂。甘蔗赤霉素不敏感(gibberellic acid insensitive, ScGAI)基因编码1个与甘蔗茎发育有关的DELLA结构域包含蛋白。本课题组前期研究发现,甘蔗ScGAI基因定位在割手密(S. spontaneum)基因组Chr 1B和Chr 1C染色体上,且受到2个不同长度的启动子调控。序列分析表明,ScGAI基因2个启动子上游1.1 kb区域的主要差异是较短的启动子在-982~-496 bp区域存在2个片段缺失。本研究通过对启动子顺式作用元件预测发现,缺失的片段包含TATA-box和CAAT-box等多个表达调控相关的顺式作用元件,推测ScGAI基因2个启动子对ScGAI存在不同的表达调控作用。构建ScGAI基因2个启动子和玉米(Zea mays)泛素(ubiquitin, UBI)基因启动子驱动β-葡萄糖苷酸酶(β-glucuronidase, GUS)基因的植物表达载体并进行拟南芥(Arabidopsis thaliana)的遗传转化。T3代转基因拟南芥不同时期和不同组织GUS染色分析表明,ScGAI基因较长的启动子在拟南芥所有组织中均有表达,而ScGAI基因较短的启动子主要在叶脉表达,同时在根和茎中弱表达。本研究为后续ScGAI基因的功能研究提供了重要的科学依据。
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柴哲
方金兰
黄润
黄翠霖
姚伟
张木清
关键词 甘蔗甘蔗赤霉素不敏感(ScGAI)基因DELLA结构域包含蛋白启动子β-葡萄糖苷酸酶(GUS)组织表达    
Abstract:Sugarcane (Saccharum officinarum) is an allopolyploid crop, and its complex genetic background leads to a more complex regulation mechanism of sugarcane genes. The ScGAI (sugarcane gibberellic acid insensitive) gene encodes a DELLA protein involved in sugarcane stem development. Previous studies of our research group's found that the ScGAI gene is located on Chr 1B and Chr 1C chromosomes of S. spontaneum genome, and is regulated by 2 promoters with different lengths. Sequence analysis showed that the main difference in the upstream 1.1 kb regions of the 2 promoters was that compared with the more extended promoter, there were 2 fragment deletions in the -982~-496 region of the shorter promoter. The prediction of cis-acting regulatory elements in this study found that the deleted fragment contained multiple cis-acting elements related to expression regulation, such as TATA-box and CAAT-box. Thus, the 2 promoters of the ScGAI gene might have different regulation effects on ScGAI expression. The plant expression vectors of the β-glucuronidase (GUS) gene driven by the promoters of the ScGAI gene or maize (Zea mays) ubiquitin (UBI) gene were constructed and transferred into Arabidopsis thaliana. GUS staining analysis of T3 transgenic Arabidopsis thaliana at different growth stages and tissues showed that the longer ScGAI gene promoter was expressed in all tissues of Arabidopsis thaliana. In contrast, the shorter ScGAI gene promoter was mainly expressed in leaf veins and weakly expressed in roots and stems. This study provides important scientific basis for the subsequent functional study of the ScGAI gene.
Key wordsSugarcane    Sugarcane gibberellic acid insensitive (ScGAI) gene    DELLA motif containing protein    Promoter    β-glucuronidase (GUS    Tissue expression
收稿日期: 2022-08-19     
ZTFLH:  S566.1  
基金资助:国家糖料产业技术体系甘蔗抗病品种选育岗位科学家项目(CARS170109)
通讯作者: * zmuqing@163.com   
引用本文:   
柴哲, 方金兰, 黄润, 黄翠霖, 姚伟, 张木清. 甘蔗ScGAI基因两个启动子的组织特异性表达分析[J]. 农业生物技术学报, 2023, 31(11): 2254-2262.
CHAI Zhe, FANG Jin-Lan, HUANG Run, HUANG Cui-Lin, YAO Wei, ZHANG Mu-Qing. Tissue specific Expressions Analysis of Two Promoters of Sugarcane (Saccharum officinarum) ScGAI Gene. 农业生物技术学报, 2023, 31(11): 2254-2262.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.11.004     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I11/2254
 
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