Cloning of the ZlPIN1a Gene and its Expression Analysis in Stem Development of Zizania latifolia Induced by Infection of Ustilago esculenta
YU Yue*, ZHOU Mei-Qi* , QI Xiao-Qing , LIU Jin-Xi , ZHANG Ya-Fen , YE Zi-Hong , CUI Hai-Feng**
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou
310018, China
Abstract：Zizania latifolia stem swelling is caused by infection of Ustilago esculenta, following the inhibition of apical dominance of the stem, which may be related to the regulation of polar transportation of auxin. In this research, 6 ZlPINs (Z. latifolia PIN formed) of indole-3-aceticacid (IAA) polar transport vector related genes were cloned from Jiaobai 'Zhejiao 7'. The Mem_trans structural domains were found among the 6
ZlPINs. Phylogenetic tree analysis showed that ZlPIN1a, ZlPIN1b, ZlPIN2 and ZlPIN3a were highly similar to wild rice (Oryza brachyantha) with the similarity of 96.80%, 89.27%, 90.52% and 82.88% respectively. The expression of different tissues of Z.latifolia showed that all of the 6 ZlPINs were significantly higher expressed in root than those in leaf and sheath (P＜0.05). The expression of ZlPIN1a, ZlPIN1b and ZlPIN4 could be detected in the stem, while the expression level of ZlPIN1a was significantly higher than other ZlPINs genes (P＜0.05) . It was speculated that ZlPIN1a would be the major regulatory gene for the polar transport of IAA in the stem of Z. latifolia. The cloned sequence of ZlPIN1a (GenBank No. om782294) had a total length of 1 770 bp and encoded 589 amino acids, including 6 exons and 5 introns. Based on the infection of U. esculenta in Z. latifolia in vitro, it was showed that the expression of ZlPIN1a in stem was inhibited by the infection of U. esculenta (P＜0.05). Compared to Male Jiaobai, the significantly higher expression of ZlPIN1a in stem were found in Normal Jiaobai and Grey Jiaobai (P＜0.05). During the development of swollen stem, the expression of ZlPIN1a in Normal Jiaobai increased firstly and then decreased, however, the expression of ZlPIN1a increased continuously in Grey Jiaobai. The expression change of ZlPIN1a would be related to the 2 stem swollen development phenotypes induced by the infection of U. esculenta. In this research, ZlPIN1a was screened as the IAA polar transport related gene expressed in the stem of Z. latifolia, which could be inhibited by the infection of U. esculenta, The expression mode of ZlPIN1a in the development of swollen stem was
discussed, which could provide a new idea for the regulation mechanism of swollen stem caused by the infection of U. esculenta in Z. latifolia.
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