Identification and Functional Study of NF-YB Family Genes in Maize (Zea mays)
LIU Jian-Han2,*, MA Yu-Xin1,2,*, SUN Yu-Tong2, FAN Zi-Xuan2, XING Ji-Hong1,2,**, ZHANG Kang1,2,**
1 State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China; 2 Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, China
Abstract：Nuclear transcription factor Y (NF-Y) is composed of 3 subunits, NF-YA, NF-YB, and NF-YC, and can specifically recognize CCAAT-BOX. In order to identify the members of NF-YB family in maize (Zea mays) and analyze their expression patterns in different tissues and physiological conditions, the NF-YB coding genes in maize genome were systematically identified and analyzed by bioinformatics methods. The expression patterns of NF-YB family members under hormone treatment were determined by spraying inbred line B73 plants with salicylic acid, jasmonic acid, and abscisic acid. The results showed that there were 18 members of the maize NF-YB gene family, all of which had NFYB domains and upstream binding sites for salicylic acid, jasmonic acid and abscisic acid. The experiment proved that the expression levels of maize NF-YB family genes increased significantly within 24 h after salicylic acid, jasmonic acid and abscisic acid treatment. It was predicted that NF-YB was involved in salicylic acid, jasmonic acid and abscisic acid pathway. Under cold, heat and UV stresses, the overall expression levels of family genes were down regulated; under salt and drought stress, the overall expression levels of family genes were increased. Under the infection of Fusarium graminearum, the expression levels of Zm00001d042196, Zm00001d010574 and Zm00001d019101 were increased. In this study, the maize NF-YB family genes were systematically identified and analyzed, the members of the maize NF-YB family were clarified, and the expression rules of maize NF- YB family members in maize biotic and abiotic stresses were revealed. This study provides an important theoretical basis for further elucidating the function of this family.
刘建涵, 马宇馨, 孙雨彤, 樊梓萱, 邢继红, 张康. 玉米 NF-YB 家族基因的鉴定与功能研究[J]. 农业生物技术学报, 2023, 31(6): 1111-1121.
LIU Jian-Han, MA Yu-Xin, SUN Yu-Tong, FAN Zi-Xuan, XING Ji-Hong, ZHANG Kang. Identification and Functional Study of NF-YB Family Genes in Maize (Zea mays). 农业生物技术学报, 2023, 31(6): 1111-1121.
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