Establishment and Phenotypic Analysis of ARL15 Knockout Mice (Mus musculus) Model
XUE Ming-Ming1, WANG Ke-Jun2, CHAN Shu-Heng1, LUO Ya-Biao1, FANG Mei-Ying1,*
1 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2 College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
Abstract：A massive number of genome wide association studies have found that ADP-ribosylation factor-like 15 (ARL15) variation is significantly associated with energy metabolism-related diseases. To investigate the biological function of this gene, CRISPR/Cas9 technique was used to construct ARL15 knockout mice (Mus musculus) model for the first time, and its phenotype was analyzed. First-generation sequencing results showed that 1 841 bp of ARL15 was knocked out, including exon 2. Compared with wild-type mice, the mRNA and protein expression levels of ARL15 were significantly decreased in ARL15-/- knockout mice (P<0.05). The embryo survival ratio of ARL15+/+, ARL15+/- and ARL15-/- mice on embryo 16.5 d was present at Mendelian Genetics; ARL15-/- mice had lower body weight on postnatal 0.5 d (P<0.05), and died shortly. qPCR showed that deletion of ARL15 inhibited adiponectin, C1Q and collagen domain containing (Adipoq) and diacylglycerol O-acyltransferase 2 (DGAT2) mRNA expression levels in fat and intestinal tissues of mice, respectively (P<0.05). In conclusion, ARL15 plays an important role in the early postnatal survival and lipid metabolism in mice. The ARL15 knockout mouse model has been successfully established, which can be used to further study the function of ARL15.
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