Abstract：A total of 34 weaning piglets from six litters were randomly assigned into two groups, offered basal diet(control) and basal diet plus manno-oligosaccharide (MOS), respectively. Faecal samples were collected on day 1(before first feeding), 7, 14, 21 and 28 after weaning. DNA was extracted from the faecal samples and the V6～V8 regions of bacterial 16S rDNA were amplified. Amplicons were analyzed by DGGE to investigate the diversity of the bacterial communities of weaning piglets. Results showed that piglets from the same litter had a higher similarity(81%~88%) of the bacterial community on day 1(before weaning) compared with that after weaning(61%~70%). DGGE similarities of microbial community between neighboring time points, e.g. 1~7, 7~14, 14~21 and 21~28, decreased during the first week for all piglets, but the value in the control remained around 61%, while those in the MOS treatment gradually increased to 70%. Diversity analysis showed that after 7 days the value for the MOS treatment increased while that for the control decreased. On day 28 the diversity index of the bacterial community from the MOS diet was 1.52 and the control group was 1.38. The differences of the diversity between the two groups increased from 0.09 on day 1 to 1.14 on day 28. In agreement with diversity data, DGGE band numbers revealed that during the first week of weaning the average band numbers reduced for both groups. However, after 14 days average band number for piglets fed the MOS diet increased, while that from piglets fed the control diet decreased. The results demonstrated that weaning had caused rapid changes of the bacterial community of piglets and that MOS supplemented in the diet could result in a rapid development and consequently early establishment of a high diverse bacterial community after weaning.
[J]. , 2006, 14(5): 701-705.
HANG Su-qin;MAO Sheng-yong;HUANG Rui-hua;SU Yong;ZHU Wei- yun. Effect of Mannan-oligosaccharide on Bacterial Community of Piglet after Weaning . , 2006, 14(5): 701-705.
