Identification of Spot Blotch Resistance in Barley (Hordeum vulgare) and Genome-wide Association Study
QU Jie-Qiong1,2, ZHANG Yi1,2, YANG Qing-Li1,2, WANG Jun-Cheng1,2, YAO Li-Rong1,2, SI Er-Jing1,2, MA Xiao-Le1,2, LI Bao-Chun1,3, FANG Yong-Feng1,2, WANG Hua-Jun1,2, MENG Ya-Xiong1,2,*
1 State Key Laboratory of Aridland Crop Science, Gansu Agricultural University/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, China; 2 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China; 3 College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Barley (Hordeum vulgare) is an important crop and industrial raw material, and spot blotch is one of the diseases that seriously endanger barley production. The disease mainly damages leaves and leaf sheaths, and its occurrence and prevalence seriously affect the yield and quality of barley. In order to explore the molecular markers related to barley spot blotch resistance, 142 barley materials were identified by artificial inoculation of spot blotch pathogen at seedling stage, and SSR molecular markers combined with phenotypic identification results of barley resistance to spot blotch were used for correlation analysis, so as to find effective resistance sites. The results showed that 504 alleles were detected from 70 SSR markers, with an average of 7.2 alleles per marker. The variation range of major allele frequency was 0.196 3 to 0.503 5, with an average of 0.344 2, and the variation range of PIC was 0.521 2 to 0.847 0. The genetic diversity index ranged from 0.600 7 to 0.862 4, and the population was divided into 5 groups by cluster analysis and population genetic structure analysis. A total of 6 SSR markers were detected, which were located on the 3H, 4H and 5H chromosomes of barley, respectively, and a QTL locus that confered resistance to barley spot blotch was finally determined on the 4H chromosome. The results of this study will be helpful for the breeding of barley variety, and the associated loci of barley spot blotch resistance obtained can provide reference for molecular marker-assisted breeding of barley.
曲洁琼, 张毅, 杨庆丽, 汪军成, 姚立蓉, 司二静, 马小乐, 李葆春, 方永丰, 王化俊, 孟亚雄. 大麦种质抗叶斑病鉴定和全基因组关联分析[J]. 农业生物技术学报, 2022, 30(12): 2267-2278.
QU Jie-Qiong, ZHANG Yi, YANG Qing-Li, WANG Jun-Cheng, YAO Li-Rong, SI Er-Jing, MA Xiao-Le, LI Bao-Chun, FANG Yong-Feng, WANG Hua-Jun, MENG Ya-Xiong. Identification of Spot Blotch Resistance in Barley (Hordeum vulgare) and Genome-wide Association Study. 农业生物技术学报, 2022, 30(12): 2267-2278.
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