Development and Validation of SSR Markers Closely Linked to Thin Shell in Tartary Buckwheat (Fagopyrum tataricum)
CHEN Zheng-Feng1, XUE Xian-Bin1, LI Rui-Yuan2, JIA Qiong1, CHEN Qing-Fu1, SHI Tao-Xiong1,*
1 Research Center of Buckwheat Industry Technology, Guizhou Normal University, Guiyang 550001, China; 2 Key Laboratory of Information and Computing Science of Guizhou Province, Guiyang 550001, China
Abstract：Tartary buckwheat (Fagopyrum tataricum) is one of the important coarse grain crops. Different shell types lead to different hulling efficiency, which has great influence on the development of Tartary buckwheat industry. At present, there are few molecular markers study on Tartary buckwheat shell types. The development of molecular markers is helpful to judge the types of Tartary buckwheat shells, and also provides reference for the improvement of Tartary buckwheat shell characteristics and selection of Tartary buckwheat germplasm. In this study, SSR markers were developed based on the loci interval of Tartary buckwheat thin shell gene traits. The results showed that a total of 286 SSRs were detected in the 1.4 Mb mapping interval of thin-shell type. Mono- and di-nucleotide repeat types were the most numerous, and there were 92 and 103, respectively. A/T and AT/AT is the most abundant motifs in mono-nucleotid and di-nucleotide repeat types, respectively. Repeat number of SSR types concentrated between 4 and 10 times. A total of 186 pairs of SSR primers were successfully designed, and 5 pairs of SSR markers, FtgGKssr-82, FtgGKssr-131, FtgGKssr-147, FtgGKssr-149 and FtgGKssr-175, showed polymorphism between the parents of RILs population. The linkage analyses of the phenotype marker for hull type and the 5 SSR markers in RILs population indicated that marker FtgGKssr-149 and FtgGKssr-175 were most closely linked to shell type. Correlation analysis between the shell rate and the 5 SSR markers by using 32 Tartary buckwheat varieties (lines) showed that the markers FtgGKssr-147, FtgGKssr-149 and FtgGKssr-175 were closely related to the shell rate. In this study, 3 SSR markers that were closely linked to the hull type and significantly associated with the shell rate of Tartary buckwheat could be used for marker-assisted selection of Tartary buckwheat germplasms with thin hull, which is of high importance in accelerating the breeding process of thin-hull Tartary buckwheat varieties.
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