Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica
YANG Bei-Bei1, YANG Yang2, DAI Dong-Qing1, LIU Ning3,4, JIA Hui4, CAO Zhi-Yan3,4,*, DONG Jin-Gao3,4,*
1 College of Life Science, Hebei Agricultural University, Baoding 071000, China; 2 Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China; 4 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China
Abstract：The genetic variation of Setosphaeria turcica was mainly caused by sexual reproduction. In order to explore the role of the S. turcica mating type (MAT) genes StMAT1-1 and StMAT1-2 in sexual reproduction, some strains with different mating type genes were sexually induced, and qRT-PCR was used to analyze the expression patterns of StMAT1-1 and StMAT1-2 in different mating type strains at 0, 15, 30 and 60 d after sexual induction. The transcriptional activity of StMAT1-1 and StMAT1-2 was determined by yeast (Saccharomyces cerevisiae) self-activation test. The results showed that StMAT1-1 and StMAT1-2 genes were expressed before sexual induction, 15, 30 and 60 d after sexual induction. With the extension of induction time, the expression of mating type genes in different induction combinations also changes significantly, indicating that MAT genes played an important role in the sexual reproduction process of S. turcica. In yeast self-activation test, it was found that the encoded proteins of StMAT1-1 and StMAT1-2 genes were transcription factors with high transcriptional activation activity. This study has reference value to the further research on the key role of MAT genes in the regulation of sexual reproduction of S. turcica.
杨贝贝, 杨阳, 戴冬青, 刘宁, 贾慧, 曹志艳, 董金皋. 玉米大斑病菌交配型基因StMAT1-1和StMAT1-2表达与转录活性分析[J]. 农业生物技术学报, 2021, 29(4): 764-771.
YANG Bei-Bei, YANG Yang, DAI Dong-Qing, LIU Ning, JIA Hui, CAO Zhi-Yan, DONG Jin-Gao. Expression and Transcriptional Activity Analysis of Mating Type Genes StMAT1-1 and StMAT1-2 in Setosphaeria turcica. 农业生物技术学报, 2021, 29(4): 764-771.
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