联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月3日 星期四
农业生物技术学报  2021, Vol. 29 Issue (12): 2267-2278    DOI: 10.3969/j.issn.1674-7968.2021.12.001
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
杧果DBB基因鉴定及其在胶孢炭疽菌和细菌性黑斑病菌侵染过程中的表达分析
孙宇1,2, 刘志鑫2, 叶子1,2, 高爱平3, 罗睿雄3, 蒲金基1,2, 张贺1,2,*
1海南大学 植物保护学院/热带农林生物灾害绿色防控教育部重点实验室,海口 570228;
2中国热带农业科学院 环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室,海口 571101;
3中国热带农业科学院 热带作物品种资源研究所,海口571101
Identification of Mango (Mangifera indica) DBB Genes and Its Expression Analysis During the Infection of Colletotrichum gloeosporioides and Xanthomonas campestris pv. mangiferaeindicae
SUN Yu1,2, LIU Zhi-Xin2, YE Zi1,2, GAO Ai-Ping3, LUO Rui-Xiong3, PU Jin-Ji1,2, ZHANG He1,2,*
1 Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education/College of Plant Protection, Hainan University, Haikou 570228, China;
2 Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs/Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
3 Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
全文: PDF (9914 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 B-box蛋白是一类锌指结构转录因子,其功能具有普遍性和特异性。为了揭示双B-box结构域锌指蛋白(double B-box, DBB)基因家族在杧果(Mangifera indica)中的序列特征及其表达特性,本研究以杧果全基因组数据为基础,采用生物信息学的方法对杧果DBB基因家族成员进行序列分析,并通过qRT-PCR技术研究杧果胶孢炭疽菌(Colletotrichum gloeosporioides, Cg)和细菌性黑斑病菌(Xanthomonas campestris pv. mangiferaeindicae, Xcm)侵染过程中杧果DBB基因家族成员的相对表达量。结果表明,从杧果基因组中鉴定了9个DBB基因家族成员,命名为MiDBB1~9,9个MiDBBs蛋白均为不稳定亲水酸性蛋白,主要定位于细胞核中,不仅三级结构类似,N端还具有相同位点的两个保守结构域(B-box1和B-box2)。根据蛋白结构预测、系统发育关系、保守基序及多重序列比对分析推测,杧果DBB基因家族在进化上具有结构保守性,功能上具有相似性。杧果与拟南芥(Arabidopsis thaliana)、烟草(Nicotiana tabacum)、苹果(Malus domestica)、菠萝(Ananas comosus)和毛果杨(Populus trichocarpa)构建的系统发育树显示,杧果DBB与毛果杨DBB亲缘关系最近,其次是苹果。qRT-PCR结果显示,胶孢炭疽菌侵染过程中,MiDBB1~9的相对表达量在12~48 h内持续上调,细菌性黑斑病菌侵染过程中,MiDBB2MiDBB3的相对表达量在12~72 h内持续上调,初步确定了杧果DBB基因表达受胶孢炭疽菌和细菌性黑斑病菌的诱导。本研究结果为后续研究杧果DBB基因家族成员的功能和作用机制提供参考。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
孙宇
刘志鑫
叶子
高爱平
罗睿雄
蒲金基
张贺
关键词 杧果双B-box结构域锌指蛋白(DBB)基因家族全基因组鉴定表达分析    
Abstract:B-box protein is a kind of zinc finger structure transcription factor, its function is universal and specific. In order to reveal the sequence and expression characteristics of the double B-box (DBB) gene family in Mangifera indica, in this study, based on the whole genome data of mango, using bioinformatics methods to analyze the sequence of mango DBB gene family, and the relative expression level of DBB gene family was studied by qRT-PCR during the infection of Colletotrichum gloeosporioides (Cg) and Xanthomonas campestris pv. mangiferaeindicae (Xcm). Nine DBB gene family members were identified from the mango genome and named MiDBB1~9. The 9 MiDBBs proteins were unstable hydrophilic acidic proteins, mainly located in the nucleus. Not only was the tertiary structures similar, but also the N-terminal were 2 conserved domains (B-box1 and B-box2) with the same site. According to protein structure prediction, phylogenetic relationship, conservative motifs and multiple sequence comparison analysis, it was inferred that the DBB gene family of mango was evolutionarily conserved in structure and similar in function. The phylogenetic tree constructed by mango with Arabidopsis thaliana, Nicotiana tabacum, Malus domestica, Ananas comosus and Populus trichocarpa showed that the DBB of mango were closely related to those of the P. trichocarpa, followed by apple. The results of qRT-PCR showed that during the infection of C. gloeosporioides, the relative expression of MiDBB1~9 continued to increase within 12~48 h. During the infection of X. campestris pv. mangiferaeindicae, the relative expression levels of MiDBB2 and MiDBB3 continued to increase within 12~72 h. It was preliminarily speculated that the expression of DBB gene in mangosteen was induced by C. gloeosporioides and X. campestris pv. mangiferaeindicae. The above findings provide reference for the follow-up study of the functions and mechanisms of mango DBB gene family members.
Key wordsMango    Double B-box (DBB) gene family    Genomic identification    Expression analysis
收稿日期: 2021-04-29     
ZTFLH:  S667.7  
基金资助:国家重点研发专项(2019YFD1000504); 中国热带农业科学院基本科研业务费专项资金(1630042021022; 1630042017019)
通讯作者: *atzzhef@163.com   
引用本文:   
孙宇, 刘志鑫, 叶子, 高爱平, 罗睿雄, 蒲金基, 张贺. 杧果DBB基因鉴定及其在胶孢炭疽菌和细菌性黑斑病菌侵染过程中的表达分析[J]. 农业生物技术学报, 2021, 29(12): 2267-2278.
SUN Yu, LIU Zhi-Xin, YE Zi, GAO Ai-Ping, LUO Rui-Xiong, PU Jin-Ji, ZHANG He. Identification of Mango (Mangifera indica) DBB Genes and Its Expression Analysis During the Infection of Colletotrichum gloeosporioides and Xanthomonas campestris pv. mangiferaeindicae. 农业生物技术学报, 2021, 29(12): 2267-2278.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2021.12.001     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2021/V29/I12/2267
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn