Bioinformatics and Expression Analysis of StERF109 Gene in Potato (Solanum tuberosum)
WANG Fang-Fang1, YANG Jiang-Wei1,2, ZHU Xi3, LI Shi-Gui3, LIU Wei-Gang3, ZHANG Ning1,*, SI Huai-Jun1,2
1 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China; 3 College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Ethylene responsive factors (ERFs) belong to the AP2/ERF (APETALA2/ethylene responsive factor) superfamily, which are one of the largest transcription factor families in plants. Previous studies have shown that ERFs are involved in a variety of biological processes in plants, such as growth and development, signal transduction, biotic, abiotic stresses and so on. In this study, in order to study the function of potato (Solanum tuberosum) StERF109 gene (GenBank No. XM_006355301.2), bioinformatics methods were used to analyze the StERF109 protein, and the subcellular localization fusion expression vector StERF109-EGFP was constructed to locate the StERF109 gene. The tissue-specific expression of StERF109 was analyzed using qPCR technology. The results showed that StERF109 contained a typical AP2 domain, which was a hydrophilic protein without transmembrane structure. StERF109 was located on the nucleus and cell membrane. StERF109 gene had the highest expression level in potato roots, but there were differences in the expression level in potato stems and leaves of different potato varieties. StERF109 gene was up-regulated under drought and salt stress, which indicated that StERF109 gene might played an important role in drought and salt stress. The results of the study provide a reference for further research on the function of potato StERF109 gene.
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