Abstract：Starch is one of the important quality characters of potato (Solanum tuberosum), and the development of linkage molecular markers could promote the breeding of high starch content varieties. In this study, 106 F1 individuals and 240 F2 individuals and their parents tetraploid potato wild species (S. demissum) 'YSP-4' (female parent) and low starch line 'MIN-021' (male parent) were used as materials, a candidate interval associated with starch content was excavated based on the previous results of specific-locus amplified fragment sequencing (SLAF-seq) combined bulked segregant analysis (BSA) strategy, two SSR markers chr2-SSR14 and chr2-SSR22 linked to starch content were developed in this interval, respectively, and verified by F2 segregation population. The results showed that the correlation between the detection results of chr2-SSR14 and chr2-SSR22 markers and phenotypic identification in F2 segregation population was 89.3% and 92.9%, respectively. After correlation analysis by SPSS software, the correlation coefficients (r) of the 2 markers were 0.68 and 0.769, respectively, which were significantly correlated at the level of 0.01 (P<0.01). The chr2-SSR14 and chr2-SSR22 markers developed in this study could be used for marker-assisted selection of high starch content trait in potato, which is of great significance for the breeding of new varieties of tetraploid potato with high starch content.
李佳奇, 于卓, 张胜, 于肖夏, 李景伟, 卢倩倩, 杨东升. 四倍体马铃薯淀粉含量性状相关SSR标记的开发与验证[J]. 农业生物技术学报, 2021, 29(8): 1630-1639.
LI Jia-Qi, YU Zhuo, ZHANG Sheng, YU Xiao-Xia, LI Jing-Wei, LU Qian-Qian, YANG Dong-Sheng. Development and Validation of SSR Markers Related to Starch Content Traits in Tetraploid Potato (Solanum tuberosum). 农业生物技术学报, 2021, 29(8): 1630-1639.
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