Identification and Drought Resistance Analysis of APX Gene Family in Gossypium hirsutum
CHEN Qin1,2,*, LI Duo-Lu1,*, GAO Wen-Ju1, WANG Ting-Wei1, FU Jin-Cheng1, CHEN Quan-Jia1, QU Yan-Ying1,**
1 College of Agriculture/Cotton Engineering Research Center of Ministry of Education/Key Laboratory of Agricultural Biotechnology, Xinjiang Agricultural University, Urumqi 830052, China; 2 College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi 830052, China
Abstract：Ascorbate peroxidase (APX) is a key enzyme for scavenging reactive oxygen species. It can catalyze the transformation of H2O2 to H2O and plays an important role in plant stress. In the early stage, the research group screened APX gene with sequence variation between drought resistant and drought sensitive materials through two-dimensional electrophoresis of root proteome of Gossypium hirsutum. In order to explore the drought resistance function of cotton APX gene, 18 GhAPXs were identified from Gossypium hirsutum genome in this study, named GhAPX01 to GhAPX18. Phylogenetic tree analysis showed that members of the GhAPX family were clustered into four subgroups; Cis-acting elements analysis showed that there were abscisic acid, salicylic acid and gibberellin elements upstream of most of the APX family genes; Transcriptome thermography and qPCR results showed that GhAPX07 and GhAPX16 were induced to express under drought stress, and showed different expression patterns in drought resistant and drought sensitive cotton materials. It was speculated that these two genes might play an important role in the response of cotton to drought stress. These results provide a reference for further study on the drought resistance function and molecular mechanism of GhAPX gene.
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