Identification and Expression Analysis of PIN Gene Family in Different Tree Architectures of Peach (Prunus persica)
TAN Bin1,2, YANG Li-Ping1, CHEN Li-Chuan1, FENG Mei-Qiao1, LIAN Xiao-Dong1, WEI Peng-Cheng1, CHENG Jun1,2, WANG Xiao-Bei1,2, FENG Jian-Can1,2,*
1 College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou 450002, China
Abstract：The PIN-FORMED (PIN) gene family of trans-membrane proteins as auxin efflux carriers plays a crucial role in polar auxin transport and then influences the growth and development of plants. The objective of this study is to understand the distribution, structure and evolution of PIN protein genes in peach (Prunus persica) genome, to study the expression characteristics of PIN family members in different tree architectures of peach. In this study, PpPIN family was identified from the whole peach genome according to the reported 8 PINs of Arabidopsis thaliana and Mem_trans (PF03547) conserved domains of AtPIN proteins. Several bioinformatics softwares, including TBtools v0.6733, GSDS 2.0, MEME 4.9, Protparam V12.1, MEGA 7.0 and PlantCARE were used to identify chromosome location, gene structure, physical and chemical properties of protein, motif analysis, phylogeny analysis and cis-elements of promoters on PpPIN protein family, respectively. Meanwhile, the expression characteristics of PpPIN genes in the shoot tips of different tree architecture of peach were analyzed based on the results of transcriptome sequencing. And the expression characteristics of PpPIN genes were also analyzed by real-time quantitative RT-PCR (qRT-PCR) in different tissues (organs) of different types of peach. Fifteen PpPIN genes were identified and unevenly distributed on 6 chromosomes of peach. Except for PpPIN3 and PpPIN5, all other PpPIN genes contained introns, with the number of introns ranging from 1 to 10. The number of amino acids and the isoelectric point of PpPIN proteins which were hydrophilic proteins were 357~678 and 4.97~9.40, respectively. Phylogenetic analysis results indicated the PIN proteins of 8 species, including peach, apple (Malus×domestica), Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), Populus (Populus trichocarpa), potato (Solanum tuberosum), maize (Zea mays) and soybean (Glycine max), were divided into 3 groups (canonical type, noncanonical type 1 and noncanonical type 2). According to the results of evolutionary analysis, 15 PpPIN proteins were divided into 3 groups. Canonical type, Noncanonical type1 and Noncanonical type 2 included 6, 2 and 7 PIN proteins, respectively. The number of conserved motifs of 8 PpPIN proteins of Canonical type and Noncanonical type 1 was 10, and the order of 10 motifs in these 2 types was all the same. While the number of conserved motifs of 7 PpPIN proteins of noncanonical type 2 was 8~11, and the arrangement of conserved motifs was not the same. The cis-element analysis of 15 PpPIN gene promoters showed that the members of PpPIN gene family were affected by light, temperature, drought stress and other environmental conditions, as well as abscisic acid, gibberellin and other hormones. Fifteen PpPIN genes were differentially expressed in different tree architecture of peach. According to the transcriptome analyses, the expression level of PpPIN12 (the homologous gene of AtPIN5) in the shoot tip of 'Fenhuashouxingtao' (dwarf type) was significantly higher than that of 'Qiumihong' (standard type), while the expression level of PpPIN12 in the shoot tip of 'Sahonglongzhu' (pillar type) was significantly lower than that of 'Okuba' (standard type). The expression level of PpPIN2 (the homologous gene of OsPIN2) in the upper phloem of the branch junctions of 'Okubo' (standard type) was obviously higher than that of 'Sahonglongzhu' (pillar type), while the expression level of PpPIN13 gene (the homologous gene of OsPIN13) was opposite to PpPIN2. The 3 candidate genes, including PpPIN12, PpPIN2 and PpPIN13, may play an important role in regulating the formation of plant height and branch angle of peach trees.
谭彬, 杨丽萍, 陈立川, 冯玫侨, 连晓东, 魏鹏程, 程钧, 王小贝, 冯建灿. 桃PIN蛋白基因家族鉴定及其在不同树型桃中的表达分析[J]. 农业生物技术学报, 2020, 28(10): 1747-1760.
TAN Bin, YANG Li-Ping, CHEN Li-Chuan, FENG Mei-Qiao, LIAN Xiao-Dong, WEI Peng-Cheng, CHENG Jun, WANG Xiao-Bei, FENG Jian-Can. Identification and Expression Analysis of PIN Gene Family in Different Tree Architectures of Peach (Prunus persica). 农业生物技术学报, 2020, 28(10): 1747-1760.
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