Abstract：Pluripotent stem cells have the ability of self-renewing and multiple differentiation potential. Germ cells are the carriers of genetic information. Direct differentiation of germ cells from pluripotent stem cells not only lays the foundation of studying reproductive development and organogenesis,but also provides new ideas and methods of treating human (Homo sapiens) infertility and conserving excellent animal breeds. At present,the study of induced differentiation of mouse (Mus musculus) pluripotent stem cells into germ cells is the most mature,and functional germ cells can be obtained in vitro; the system of induced differentiation of human,pig,cattle,sheep and other livestock pluripotent stem cells into germ cells is not mature,which needs further optimization. In this paper,the methods and systems of inducing differentiation of mouse,human and livestock pluripotent stem cells into germ cells are reviewed,and the problems and research strategies in inducing differentiation are prospected. This review provides a theoretical basis for the differentiation of domestic animal pluripotent stem cells into germ cells.
相金柱, 王寒凝, 李雪玲. 哺乳动物多能干细胞向生殖细胞分化的研究进展[J]. 农业生物技术学报, 2020, 28(11): 2061-2068.
XIANG Jin-Zhu, WANG Han-Ning, LI Xue-Ling. Research Progress in Differentiation of Germ Cells from Mammalian Pluripotent Stem Cells. 农业生物技术学报, 2020, 28(11): 2061-2068.
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