Genome-wide Identification of Capsicum annuum R2R3-MYB Transcription Factors and Expression Pattern Analysis in Phytophthora capsici Response
HE Wei2, ZHANG Hui1,*
1 Institute of Horticulture, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, China; 2 School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guian 550025, China
Abstract：MYB transcription factor family is one of the largest families of transcription factors in plants, which are extensively involved in many physiological and biochemical processes. In order to explore the function of MYB transcription factor family of pepper (Capsicum annuum), this study conducted genome-wide identification and bioinformatics analysis, and detected the effects of Phytophthora capsici on the transcription level of the family members by transcriptome technique. The results showed that 102 R2R3-MYB transcription factors were identified from C. annuum genome database, which were distributed on each chromosome. Phylogenetic tree analysis found that R2R3-MYB proteins of pepper could be classified into 11 subgroups, and subfamily Ⅺ had a clear distinction from other subfamily in conserved domains, shared motifs and gene structure. Conserved domains included 2 classes with significant differences, but the conserved domains in each class were highly similar. The R2R3-MYB gene structure was significantly different among the subgroups. In the pepper genome, there were 5 pairs of homologous R2R3-MYB genes from tandem replication. The R2R3-MYB genes of Arabidopsis thaliana, Solanum lycopersicum and C. annuum could be classified into 22 subgroups by phylogenetic analysis. Above results indicate that R2R3-MYB proteins initially diversified through genome replication, intron insertion and domain shuffling, but remained relatively conserved throughout the subsequent plant evolution. Further analysis of R2R3-MYB gene expression pattern using pepper transcriptome data revealed that some of them could response to P. capsici. The expression of some R2R3-MYB transcription factors were up-regulated in disease-resistant varieties, suggesting that expressions of MYB transcription factor could be induced by P. capsici. The present study could provide a reference for in-depth exploration of the mechanism of pepper R2R3-MYB transcription factor family involved in P. capsic resistance.
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