马体尺性状的全基因组关联分析

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摘要马匹(Equus caballus)体尺性状影响马运动性能,利用新技术研究马体尺性状为马匹运动性能的研究引入新方法。为丰富马体尺性状与运动性能的研究内容,探索间接调控运动性能的基因,本研究利用全基因组关联分析方法(genome-wide association study,GWAS)挖掘影响马体尺性状的遗传标记和功能基因。利用马670 K高密度芯片,对伊犁马、纯血马、俄罗斯速步马、奥尔洛夫马共4个品种157个个体的体尺性状(体高,体长,管围,尻高)及体尺指数(体长率,管围率,胸围率)进行全基因组关联分析。利用Plink1.9软件对SNP位点进行质控,质控后剩余494 137个SNP用于后续关联分析。利用Ensembl等数据库进行基因注释和基因功能分析,通过基因组水平的Bonferroni校正,从体高、管围、尻高、胸围率、管围率5个性状中分别检测到3、15、1、1和9个潜在显著关联的SNP (P<9.29E-06),未检测到与体长和体长率显著相关的SNP。结果发现泛素结合酶E2家族的第三亚家族(ubiquitin conjugating enzyme E2 E3,UBE2E3)、光蛋白聚糖(lumican,LUM)基因及其信号通路中下游基因调控马运动性能。本研究为分子标记辅助选择在马育种中的应用提供参考。

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	刘玲玲
	孟军
	王琼
	曹行
	吕士鹏
	刘武军
	姚新奎

关键词 ：体尺性状, 运动性能, 全基因组关联分析(GWAS)

Abstract：Morphometric influences sports performance,new techniques of genome-wide association study (GWAS) for morphometric trait of horse introduce new possibilities for sports performance. The aim of this study was to enrich the contents of morphometric traits and sport performance of horses (Equus caballus),it can explore the regulation genes of sport performance. In this study,GWAS methods were used to seek genetic markers and functional genes that affect the morphology traits of horses. A GWAS for morphometric traits (withers height (WH),body length (BL),cannon circumference (CC),croup height (CH),body length ratio (BLR),heart girth ratio (HGR),cannon circumference ratio (CCR) were conducted in 4 breeds of 157 individual horses (Yili horses,Thoroughbred horse,Russian speed horse and Orlov horse) which were genotyped using the Affymetrix Equine SNP 670 chip. Plink1.9 software was used to quality control for SNPs,after quality control,494 137 SNPs were participated in association analysis. Databases of Ensembl were used to carry out gene annotation and gene function analyses. Through Bonferroni correction at the genomic level,3 SNPs,15 SNPs,1 SNPs,1 SNPs and 9 SNPs were potentially significantly associated with the 5 traits of WH,CC,CH,HGR,CCR,respectively (P<9.29E-06). However,no SNPs were associated with BL and BLR. It was found that the 2 genes UBE2E3 and LUM regulated the sport performance of horses,which provided a reference for the application of marker-assisted selection in horse breeding.

Key words： Morphometric traits Sport performance Genome-wide association study (GWAS)

收稿日期: 2020-01-06

ZTFLH:

S821.9

基金资助:国家自然科学基金(31560620);新疆维吾尔自治区自然基金(2019D01B14)

通讯作者: *lwj_ws@163.com;yxk61@126.com

引用本文:

刘玲玲, 孟军, 王琼, 曹行, 吕士鹏, 刘武军, 姚新奎. 马体尺性状的全基因组关联分析[J]. 农业生物技术学报, 2020, 28(11): 1994-2001.
LIU Ling-Ling, MENG Jun, WANG Qiong, CAO Hang, LV Shi-Peng, LIU Wu-Jun, YAO Xin-Kui. Genome-wide Association Study of Morphometric Traits in Horse (Equus caballus). 农业生物技术学报, 2020, 28(11): 1994-2001.

链接本文:

http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2020/V28/I11/1994

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