Abstract：Lycoris sprengeri is perennial herbaceous plants of Amaryllidaceae, Lycoris with the red and blue multicolor flower, which is a good material for cut flower, potted flower and landscape gardening, so L. sprengeri has the very high commercial value. To enrich the germplasm resources and cultivate varieties in L. sprengeri, a R2R3-MYB transcription factor gene LsMYB5 cDNA sequence (GenBank No. MK779710) was successfully screened by using reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE). The LsMYB5 gene length was 871 bp, and the open reading frame (ORF) was 681 bp, which encoded 226 amino acids. The c-terminal of LsMYB5 protein contained conservative EAR inhibition domain (pdLNLD/ELxiG/s) and zinc finger domain (CX-(1-2), CX-(7-12), CX-(1-2)C), which may have transcriptional inhibition function. LsMYB5 gene mainly expressed in leaves and the fading period of petals, and there were significant differences in the expression of LsMYB5 in clones with different colors (P<0.05). To further analyze LsMYB5 gene function, prokaryotic expression vector of pET-30(a)-LsMYB5 was constructed and transformed into Escherichia coli BL21 (DE3), the expression of recombinant proteins was induced under the induction of isopropyl glucosinolates galactose glucoside (IPTG), and the His label protein was purified. The results will provide the theory basis for screening structural genes regulated by LsMYB5 transcription factor. which related to flower color formation in Lycoris sprengeri.
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