Isolation of HcNAC1 Transcription Factor Gene and Its Expression Patterns Under Drought Stress in Kenaf (Hibiscus cannabinus)
DENG Jie-Lou1, ZHANG Gao-Yang1,*, ZHANG Zong-Liang1, ZHANG Chao1, XU Jian-Tang2, QI Jian-Min2, WU Ying-Bao2, WANG Gang1
1 College of Life Sciences, Shangrao Normal University, Shangrao 334001, China; 2 College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Abstract：Drought is one of the abiotic limiting factors that seriously affect the growth of crops. NAC (NAM, ATAF and CUC) transcription factor is one type of transcription factors in plants, it play an important role in regulating plant growth, development and response to abiotic stress. In order to study the regulation function of NAC transcription factor in drought stress of kenaf (Hibiscus cannabinus), a NAC like gene was isolated from kenaf transcriptome database, named HcNAC1 gene (GenBank No. MT799841). The coding sequence of HcNAC1 contained 1 044 bp, encoding 347 amino acids, protein molecular weight 38.77 kD, isoelectric point was 8.78. The HcNAC1 genes with NAM/NAC family conservative area, containing about 160 amino acid conservative in the protein N-terminal structural domain, with 2 nuclear localization signal (NLS) sequence. Phylogenetic tree analysis showed that kenaf HcNAC1 had the closest evolutionary relationship with Hibiscus syriacus. The expression level in different tissues and within 24 h of drought stress was detected by reverse transcription PCR (RT-PCR) and qRT-PCR, research results showed that HcNAC1 gene was expressed in root, stem, leaf, flower and fruit, HcNAC1 gene was expressed under PEG6000 stress and had most expression level at 12 h, and then the expression level gradually decreased. HcNAC1 played an important regulatory role in response to drought stress. The study explored the correlation between HcNAC1 expression and drought stress. and could provide a reference for further research on the molecular mechanism of HcNAC1 transcription factors involved in drought stress of kenaf.
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