Abstract：WD-repeat protein, containing WD-40 motif, also known as WD40 repeat protein, which plays an essential regulating role during the abiotic stress response of plants. In this study, it was found that a kenaf (Hibiscus cannabinus) transcriptome unigene which was highly similar to the WD40 gene, and then designed the primers to carry out reverse transcription PCR amplification and obtained HcWD40-1(GenBank No.: KX711617) cDNA sequence by sanger sequencing. Bioinformatics analysis showed that the gene open reading frame was 1 356 bp, encoding 451 amino acids, and containing 7 typical WD40 domains. qRT-PCR analysis showed that HcWD40-1 gene was induced by salt and drought stress, and was induced by abscisic acid (ABA) and methyl jasmonate (MeJA). The results suggested that the HcWD40-1 gene was a key hub gene for ABA and MeJA signaling pathways, salt and drought stress responses. The results of the study provide basic data for the study of salt-tolerant, drought-resistant on ABA signal regulation network and MeJA signal regulation network of kenaf.
[1] Kronzucker H J, Coskun D, Schulze L M,et al. Sodium as nutrient and toxicant[J].Plant and soil, 2013,369：1-23.[2]Janz D, Polle A. Harnessing salt for woody biomass production[J]. Tree Physiology,2012,32：1-3[3] Zhang J L, Flowers T J, Wang S M, Mechanisms of sodium uptake by roots of higher plants[J].Plant and soil, 2010,326：45-60[4] R.Munns and M.Tester, Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008,vol.59,pp.651-681[5]Mishra AK,Puranik S,Prasad M.Structure and regulatory networks of WD40 protein in plants[J].J Plant Biochem Biot,2012,21(1)：32-39[6]Xu Min.Structure and function of WD40 domain proteins[J].Protein and Cell,2011,2(3)：202-214[7]Ramsay NA,Glover BJ. MYB-Bhlh-WD40 protein complex and the evolution of cellular diversity[J].Trends Plant Sci,2005,10(2)：63-70[8]Broun.Transcriptional control of flavonoid biosynthesis：a complex network of conserved regulators involved in multiple aspects of differentiation in Arabidopsis[J].Current Opinion in Plant Biology,2005,8：272-279[9] 游晓慧，李威，陶启长，孙小芬，唐克轩.WD40重复蛋白家族基因Atg65030调控拟南芥种子的重量与体积[J].植物生理学报，2011,47（7）：715-725[10] 李宝燕.烟草WD40蛋白TTG2对生长发育和抗病性的调控作用[M].南京：南京农业大学，2012.[11] 李辉，李德芳，陈安国等.红麻雄性不育研究进展及展望[J].作物研究，2015,29（2）206-209[12] Ji Huang,Mei-Mei Wang, Yong-Mei Bao,Shu-Jin Sun,Li-Juan Pan,Hong-Sheng Zhang.SRWD：A novel WD40 protein subfamily regulated by salt stress in rice(Oryza sativa L.) [J]. Gene 2008,424：71-79[13] 李洁.棉花GhWD40基因的克隆及功能验证[M].武汉，华中农业大学，2013[14]张春蕊，贾园园，王艳敏，王玉成.刚毛柽柳液泡膜H+-PPase基因的克隆与胁迫下的表达分析[J]. 西北植物学报，2016,36（5）：0881-0887[15]郑琳琳，张慧荣，贺龙梅，王迎春.唐古特白刺质膜Na+/H+逆向转运蛋白基因的克隆与表达分析[J]. 草业学报，2013,22（4）：179-186[16]裴斌，张光灿，张淑勇，吴芹，徐志强，徐萍.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报，2013,33（5）：1386-1396[17]曹红利，岳川，周艳华，王璐，郝心愿等.茶树bZIP 转录因子基因CsbZIP1 的克隆与表达定位[J].作物学报，2014,40（9）：1702-1709[18]马建辉，张戴静，高小龙，郝云，姜丽娜.乌拉尔图小麦WRKY转录因子的筛选与分析[J].作物学报，2015,41（6）：900-909 [19]Asano T,Hakata M,Nakamura H,etal. Functional characterisation of OsCPK21,a calcium-dependent protein kinase that confers salt tolerance in rice[J].Plant Mol Bil,2011,75(12)：179-191[20]Asano T,Hayashi N,Kobayashi M,et al.A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance[J].Plant J,2012,69(1)：26-36[21]Xu D B,Chen M,Ma Y N,et al.A G-Protein βSubunit,AGB1,negatively regulates the ABA response and drought tolerance by down-regulating AtMPK6-related pathway in Arabidopsis[J].PloS One,2015,10：e0116385 [22] 李长宁，Mano J Kumar SRIVAATAVA 农倩，李杨瑞.水分胁迫下外源ABA提高甘蔗抗旱性的作用机制[J].作物学报，2010,5：863-870[23] 王允，张逸，刘灿玉，张志焕等.干旱胁迫下外源ABA对姜叶片活性氧代谢的影响[J].园艺学报，2016,43（3）：587-594[24] 陈涛，王贵美，沈伟伟，李小珍，祁建民等.盐胁迫对红麻幼苗生长及抗氧化酶活性的影响[J].植物科学学报，2011,29（4）：493-501
