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2025年4月3日 星期四
农业生物技术学报  2019, Vol. 27 Issue (6): 1090-1100    DOI: 10.3969/j.issn.1674-7968.2019.06.015
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
禾谷镰孢菌过氧化物酶家族基因的表达规律分析
柳健虎*, 张康*, 藏金萍, 曹宏哲, 张靖, 邢继红*, 董金皋*
河北农业大学 生命科学学院/河北省植物生理与分子病理学重点实验室/河北农业大学 真菌毒素与植物分子病理学实验室,保定 071000
Expression pattern Analysis of Peroxidase Family Genes in Fusarium graminearum
LIU Jian-Hu*, ZHANG Kang*, ZANG Jin-Ping, CAO Hong-Zhe, ZHANG Jing, XING Ji-Hong**, DONG Jin-Gao**
College of Life Sciences, Hebei Agricultural University/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology/Mycotoxin and Molecular Plant Pathology Laboratory of Hebei Agricultural University, Baoding 071000, China
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摘要 过氧化物酶作为病原真菌抗氧化防御系统的主要组分,能够清除来源于植物体产生的活性氧(reactive oxygen species, ROS),促使病原菌成功侵染宿主植物细胞,禾谷镰孢菌(Fusarium graminearum)过氧化物酶(peroxidase,POX)的相关研究尚未见报道。本研究利用生物信息学手段搜索真菌过氧化物酶基因的数据库,获得了31个禾谷镰孢菌过氧化物酶基因,系统进化分析和保守结构域分析均将其分为18个亚家族。对过氧化物酶家族基因的表达模式进行分析,发现FgPOX (禾谷镰孢菌过氧化物酶, Fusarium graminearum peroxidase)-13FgPOX-17FgPOX-16FgPOX-19等基因在菌丝和分生孢子中均具有较高表达水平,FgPOX-24FgPOX-29在菌丝中表达水平较高,FgPOX-11FgPOX-10FgPOX-4FgPOX-23在分生孢子中表达水平较高。对过氧化物酶家族基因在侵染过程中的表达规律进行分析,发现FgPOX-13在病菌侵染过程中表达水平明显升高并保持较高水平,FgPOX-17FgPOX-28FgPOX-30等基因在病菌侵染的早期表达水平较高,FgPOX-12、FgPOX-10、FgPOX-23等基因在病菌侵染的后期表达水平较高。进一步利用qRT-PCR技术对禾谷镰孢菌过氧化物酶家族基因在H2O2胁迫条件下表达规律进行分析,发现FgPOX-1FgPOX-4FgPOX-6FgPOX-9FgPOX-10FgPOX-22FgPOX-30的表达水平是正常条件下的20倍以上,FgPOX-9FgPOX-13等随着H2O2胁迫时间的延长,表达水平明显增高,而FgPOX-1FgPOX-21等随着H2O2胁迫时间的延长,表达水平明显降低。本研究明确了禾谷镰孢菌过氧化物酶家族基因数量、系统进化关系、保守结构域,以及在病菌不同组织、侵染过程和响应H2O2胁迫中的表达规律,为阐明禾谷镰孢菌过氧化物酶家族基因的功能提供了基础资料。
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柳健虎
张康
藏金萍
曹宏哲
张靖
邢继红
董金皋
关键词 禾谷镰孢菌过氧化物酶家族基因系统进化基因表达    
Abstract:As a main component of the anti-oxidant defense system of pathogenic fungi, peroxidase can remove reactive oxygen species (ROS) derived from plant organisms, and promote the successful infection of host plant cells by pathogens. Related studies on peroxidase (POX) of Fusarium graminearum have not been reported. In this study, 31 Fusarium graminearum peroxidase genes were obtained by searching the database of fungal peroxidase genes using bioinformatics methods, and were divided into 18 subfamilies by phylogenetic analysis and conserved domain analysis. Analysis of the expression pattern of the peroxidase family gene revealed that the FgPOX (Fusarium graminearum peroxidase)-13, FgPOX-17, FgPOX-16, FgPOX-19 and other genes had high expression levels in both mycelium and spore. and the FgPOX-24 and FgPOX-29 had higher expression levels in mycelium, the FgPOX-11, FgPOX-10, FgPOX-4 and FgPOX-23 had higher expression levels in spore. The expression pattern of peroxidase family gene in the process of infection was analyzed. It was found that the expression level of FgPOX-13 was significantly increased and maintained at a high level during the infection of the pathogen. In addition, genes such as FgPOX-17, FgPOX-28 and FgPOX-30 were highly expressed in the early stage of pathogen infection, and genes such as FgPOX-12, FgPOX-10 and FgPOX-23 were highly expressed in the late stage of pathogen infection. Furthermore, qRT-PCR was used to analyze the expression of Fusarium graminearum peroxidase family genes under H2O2 stress, the expression levels of FgPOX-1, FgPOX-4, FgPOX-6, FgPOX-9, FgPOX-10, FgPOX-22 and FgPOX-30 were found to be 20 times higher than that under normal conditions, and FgPOX-9, FgPOX-13, etc. with the prolongation of H2O2 stress time, the expression level was significantly enhanced, while FgPOX-1, FgPOX-21, etc. with the prolongation of H2O2 stress time, the expression level was reduced. This study clarified the number of genes, phylogenetic relationships, conserved domains of Fusarium graminearum peroxidase family, and the expression patterns of different tissues, infection processes and H2O2 stress in pathogens, which could provide basic datas for clarifying the function of Fusarium graminearum peroxidase family genes.
Key wordsFusarium graminearum    Peroxidase family genes    Phylogenetics    Gene expression
收稿日期: 2018-10-27     
ZTFLH:  S435.131.4  
基金资助:国家重点研发计划-粮食丰产增效科技创新专项(No. 2016YFD0300704);国家玉米产业技术体系(CARS-02-12)
通讯作者: xingjihong2000@126.com;dongjingao@126.com   
引用本文:   
柳健虎, 张康, 藏金萍, 曹宏哲, 张靖, 邢继红, 董金皋. 禾谷镰孢菌过氧化物酶家族基因的表达规律分析[J]. 农业生物技术学报, 2019, 27(6): 1090-1100.
LIU Jian-Hu, ZHANG Kang, ZANG Jin-Ping, CAO Hong-Zhe, ZHANG Jing, XING Ji-Hong, DONG Jin-Gao. Expression pattern Analysis of Peroxidase Family Genes in Fusarium graminearum. 农业生物技术学报, 2019, 27(6): 1090-1100.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.06.015     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I6/1090
 
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