Abstract：The testes are the most important reproductive organs in male mammals as they are the site of testosterone production and spermatogenesis. Normal testicular structure and physiology are the basis for reproductive activity. Proteomics is one of main methods to study proteome at post-genomics era, which may reflect the dynamic nature of proteins in whole tissue or cell. Thus it is of great significance to explore the protein compositions of testis by proteomics techniques. In this review, we mainly focus on the technological means of quantitative proteomics, as well as its application in the studies of testicular development, and responses to diseases conditions, which provides insight into the further research on genes and regulatory networks that control testicular development and size.
