Abstract：Galectins are a family of β-galactoside-binding proteins that widely distribute among insect species which play an important role in insect immune regulation. Thus the research of Aedes aegypti galectins is of great significance. In this study, primers were designed based on the A. aegypti galectin12 gene sequence, and the entire coding region of galectin12 gene was amplified by qRT-PCR with extracted total RNA. PCR product was purified and ligated to pMD-18T cloning vector. The selected positive clone was used for digested analysis and nucleotide sequencing. After endonucleases digestion, fragments were connected to pET-32a vector and obtained 23 kD protein. In addition, the expressed protein was purified by Ni-NTA affinity chromatography, and obtained purified protein bioassay result indicated that galectin12 could supporess mosquito Bt toxies. Cloning, expression and purification of Galectin 12 would help to furtherly understand the mechanism of galectin against Cry toxin for A. aegypti.
