Abstract：Abstract: Spodoptera litura multicapsid nucleopolyhedrovirus bjdp (baculovirus J domain protein ) gene is the only gene encoding a J domain protein homology in known baculovirus genome. Sequence analysis indicated that the N terminus of the gene production had a conserved J domain. With the specific primers which designed on bjdp gene reported recently, the coding region was amplified from SpltMNPV genome DNA via PCR . The PCR product was cloned into the pGEM-T Easy vector to get the recombinant plasmid (pGT-B). The bjdp gene was recombined in vitro with expression vector pQE30 and transformed into E.coli M15[pREP4]. The M15[pREP4] containing bjdp recombinant plasmid expressed a 36 kD 6×His-tag fusion protein after the induction with 1mmol/L IPTG. The fusion protein was purified with Ni-NTA resin column and used as the immunogen to raise a BJDP-specific antiserum. Western blot analysis indicated that the antiserum could react with the corresponding protein existed in SpltMNPV-infected S1-zsu-1 cells.
王立华 余健秀 尹 崇 李朝飞 庞 义**. 杆状病毒J 结构域蛋白基因的克隆、表达和抗体的制备[J]. , 2003, 11(1): 79-82.
Wang Lihua Yu Jianxiu Yin Chong Li Zhaofei Pang Yi**. Cloning and Expression of a Baculovirus J Domain Protein Gene in E. coli and Preparation of Antiserum. , 2003, 11(1): 79-82.
