Abstract：Abstract:To express foreign proteins in chicken (Gallus gallus ) oviduct cells and secret to oviduct, 450 bp chicken lysozyme cDNA fragment was cloned by using RT-PCR method. Treated by the restriction endonuclease Bam HⅠand Hind Ⅲ, the fragment was ligated with the vector generated by the same Bam HⅠ-HindⅢ digestion of plasmid pOVA-GFP. The resulting expressive vector pOVALG included an 1.2 kb chicken ovalbumin 5'-flanking regulatory region and a lysozyme-GFP fusion gene with the signal peptide of the lysozyme. After transfecting the vector to the chicken embryo fibroblasts and mammary cancer cell line of rat SHZ-88 cells, green fluorescent protein had been detected successfully.
孙明军 赵 晨 沙 金 韩海棠 郑克俭 李赞东**. 鸡输卵管组织特异性分泌表达载体的构建和表达[J]. , 2003, 11(1): 75-78.
Sun Mingjun Zhao Chen Sha Jin Han Haitang Zheng Kejian Li Zandong** . Construction and Expression of the Specific Secretory Vector in the Chicken Oviduct Cells. , 2003, 11(1): 75-78.
