Abstract：Nitrogen is the most important element affecting the yield and quality of rice (Oryza sativa). In the process of rice N-efficient breeding, molecular marker-assisted selection for target genes is helpful for efficient and convenient screening of N-efficient rice varieties. Transcription factor TCP (TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTORS) is involved in the regulation of various developmental processes. OsTCP19 is a newly reported nitrogen-efficient gene with 29 bp deletion in its promoter region. In this study, Pro TCP19-F/R was designed to develop molecular markers to identify rice materials. By comparing and analyzing the OsTCP19 promoter region of 33 reference genomic materials, it was found that 29 bp deletion of OsTCP19 promoter region was found in 'Tumba', 'CG14', 'IR64', 'N22', 'Shuhui 548', 'Basmati' and 'ZH11'. Using 'Shuhui 548' as positive control, OsTCP19 genotypes of 23 rice breeding parents, F1 generation and its paternity, and 'Xikeshui 646' hybrid lines were identified. The results showed that 'Xikeshui1288', 'Kasalath', 'IRAT109' had a deletion of 29 bp, which was consistent with 'Shuhui548' and identified as ostcp19 mutant genotype. The test-cross F1 generation of 'Xikehui1288' and 'Xikehui646' contained 2 strips, which were identified as heterozygous type. Two genotypes could be identified by single plant identification of 'Xikehui646'. Therefore, the marker identification method could identify OsTCP19 genotype and seed purity efficiently and accurately, and the identification method is simple, fast and low cost, which has potential popularization value in rice breeding.
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