Studies on Purification of Zizania latifolia Seedlings Based on the Detection of T-type Ustilago esculenta
PENG Hui1,*, YAO Liang-Hong2,*, ZHANG Zhen2, XIA Wen-Qiang1, LI Shi-Yu1, TANG Jin-Tian1, ZHANG Ya-Fen1, CUI Hai-Feng1, YE Zi-Hong1,**
1 College of Life Sciences/Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China; 2 Wuzhen Agricultural Economic Service Center, Tongxiang 314501, China
Abstract：Jiaobai is an edible fleshy stem formed by the stem of Zizania latifolia infected by Ustilago esculenta. Gray Jiaobai often appears in field production, which seriously affects the yield and quality of Jiaobai. The infection and proliferation of T-type U. esculenta is the main factor causing gray Jiaobai, normal Jiaobai was formed by the infection of MT type strain, and long-term asexual breeding makes T-type strain and MT strain coexist in Z. latifolia seedlings. Therefore, it is very important for the development of Z. latifolia industry to purify the strains in Z. latifolia seedlings and eliminate T-type strain, so as to control the production of gray Jiaobai from the source. A specific detection method for T-type strain was established, and the detection limit was 0.008 8 ng/µL. Then, through the detection of the relative content of T-type strains in seedlings under different seedling raising methods, it is clear that the shell seedling of "with Jiaobai" seedling was the best way of initial purification, and the seedling raising in the upper part of bolt tube was the best way of seedling expansion. Based on the fact that the adaptability of T-type strain to stress was much higher than that of MT-type strain, finally, a small number of purified Z. latifolia seedlings without gray Jiaobai were obtained by 5 years stress treatment of Z. latifolia seedlings and the detection of T-type U. esculenta. The above results have theoretical and practical guiding significance for the breeding of Jiaobai seedlings.
彭辉, 姚良洪, 张真, 夏文强, 李士玉, 汤近天, 张雅芬, 崔海峰, 叶子弘. 基于 T 型菰黑粉菌检测的茭白种苗纯化研究[J]. 农业生物技术学报, 2023, 31(8): 1757-1766.
PENG Hui, YAO Liang-Hong, ZHANG Zhen, XIA Wen-Qiang, LI Shi-Yu, TANG Jin-Tian, ZHANG Ya-Fen, CUI Hai-Feng, YE Zi-Hong. Studies on Purification of Zizania latifolia Seedlings Based on the Detection of T-type Ustilago esculenta. 农业生物技术学报, 2023, 31(8): 1757-1766.
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