Abstract:Glucagon-like peptide-2 receptor (GLP2R) is one of the key mediators of regulating body energy balance, which may have important effects on organ development and growth performance of sheep (Ovis aries). This study was conducted to investigate the association between GLP2R single nucleotide polymorphism and slaughter performance and weight of internal organs such as heart, liver, spleen, lung and kidney in Hu sheep. Firstly, the expression of GLP2R gene in different tissues was analyzed by qPCR. Furthermore, the polymorphism of GLP2R gene locus was detected by kompetitive allele specific PCR (KASP) typing technique in 1 351 male Hu lamb with complete pedigree, and the correlation analysis was conducted with the slaughter traits and weight of internal organs of lambs. The results showed that GLP2R gene was widely expressed in various tissues of Hu sheep, and the relative expression level was significantly higher in kidney and rumen tissues (P<0.05). There were 3 genotypes GG, GA and AA at g.34409249 G>A locus of GLP2R gene exon, which showed moderate polymorphism (0.25<PIC<0.50). This locus was not in the Hardy Weinberg equilibrium state in the population (P<0.05). The single nucleotide polymorphism of GLP2R gene was closely related to the heart, lung and kidney weight of Hu sheep, and the heart, lung and kidney weight of GG genotype group was significantly higher than that of AA genotype group (P<0.05). In conclusion, the mutation site of GLP2R gene exon in G.34409249 G>A can be used as a candidate molecular marker site for heart, lung and kidney organ development, which provides a theoretical basis for the breeding of Hu sheep.
黄永亮, 王维民, 汪晓娟, 王国秀, 马宗武, 崔盼盼, 张德印, 李冲. 湖羊GLP2R基因多态性及其与内脏重量的关联分析[J]. 农业生物技术学报, 2023, 31(2): 341-349.
HUANG Yong-Liang, WANG Wei-Min, WANG Xiao-Juan, WANG Guo-Xiu, MA Zong-Wu, CUI Pan-Pan, ZHANG De-Yin, LI Chong. Polymorphism of GLP2R Gene and Its Association Analysis with Visceral Weight in Hu Sheep (Ovis aries). 农业生物技术学报, 2023, 31(2): 341-349.
[1] 柴玉兰. 2016. 猪GLP2R和MYO6基因cDNA克隆、SNP筛选及其与部分生长性状关联研究[D]. 硕士学位论文, 湖南农业大学, 导师: 马海明, pp. 1-5, 22-28. (Chai Y L.2016. cDNA cloning, SNP screening of GLP2R and MYO6 genes and it association with partial growth traits in the porcine[D]. Thesis for M.S., Hunan Agricultural University, Supervisor: Ma H M, pp. 1-5, 22-28.) [2] 程娜. 2021. 人类致病同义突变集成学习预测方法研究及其数据库构建[D]. 博士学位论文, 安徽大学, 导师: 夏俊峰, pp. 1-35. (Cheng N.2021. Development of ensemble learning-based prediction method and comprehensive database for deleterious synonymous mutation in the human genome[D]. Thesis for Ph.D., Anhui University, Supervisor: Xia J F, pp. 1-35.) [3] 韩铖星, 张成新, 李勇, 等. 2021. 湖羊羔羊早期断奶前后胃肠道发育, 酶活性及发酵参数的变化研究[J]. 中国畜牧兽医, 48(12): 4442-4450. (Han C X, Zhang C X, Li Y, et al.2021. Changes of gastrointestinal tract development, enzyme activities and fermentation parameters of hu sheep before and after early weaning[J]. China Animal Husbandry And Veterinary, 48(12): 4442-4450.) [4] 韩宇飞, 闵伟, 徐泽立. 2014. 湖羊全舍饲方式生产状况调查研究报告[C]. 第十一届(2014)中国羊业发展大会论文集. 中国畜牧业协会, pp: 109-114. (Han Y F, Min W, Xu Z L.2014. Research report on the production status of lake sheep in full house feeding mode[C]. Proceedings of the 11th (2014) China Sheep Industry Development Conference. China Animal Husbandry Association, pp. 109-114.) [5] 梁祎凡, 曹阳, 金花子, 等. 2019. 绵羊GLP2R基因多态性及其与肉质性状的相关分析[J]. 中国畜牧兽医, 46(11): 3316-3322. (Liang Y F, Cao Y, Jin H Z, et al.2019. Correlation analysis between GLP2R gene polymorphism and meat quality traits in sheep[J]. Journal of China Animal Husbandry and Veterinary, 46(11): 3316-3322.) [6] 刘理想, 孙大明, 毛胜勇, 等. 2018. 胰高血糖素样肽-2对羔羊胃肠道重量、瘤胃发酵及小肠上皮发育相关基因表达的影响[J]. 畜牧兽医学报, 49(03): 549-558. (Liu L X, Sun D M, Mao S Y, et al.2018. Effects of glucagon-like peptide-2 on gastrointestinal weight, rumen fermentation and expression of small intestinal epithelial development-related genes in lambs[J]. ChineseJournal Of Animal Science and Veterinary medicine, 49(03): 549-558.) [7] 潘洋. 2018. 湖羊规模化养殖模式、生长发育与繁殖性能的研究[D]. 硕士学位论文, 西北农林科技大学, 导师: 曾文先, pp. 1-10. (Pan Y.2018. Study on the made of large scale farming, development and reproduction in Hu sheep[D]. Thesis for M.S., Northwest A&F University, Supervisor: Zeng W X, pp. 1-10.) [8] 尚婧晔, 朱晓露, 申红卫, 等. 2013. 胰高血糖素样肽2含量与2型糖尿病相关性的初步探究[J]. 现代预防医学, 40(09): 1739-1740, 1745. (Shang J Y, Zhu X L, Shen H W, et al.2013. The relationship between glucagon-like peptide 2 content and type 2 diabetes mellitus[J]. Modern Preventive Medicine, 40(09): 1739-1740, 1745.) [9] 孙国权, 高树新, 吴慧光, 等. 2014. 解偶联蛋白1, 2和3基因在中国西门塔尔牛组织器官中的表达水平及其与胴体品质关系分析[J]. 华北农学报, 29(4): 5. (Sun G Q, Gao S X, Wu H G, et al. 2014. Expression of uncoupling protein 1, 2 and 3 genes in bovine tissues and their relationship with carcass quality in Chinese simmental cattle[J]. Chinese Journal of Agricultural Sciences, 29(4): 5.) [10] 王东旭, 林超, 万家余, 等. 2011. SPRN转基因小鼠脏器质量和动物行为学的比较分析[J]. 中国兽医学报, 31(04): 614-618. (Wang D X, Lin C, Wan J Y, et al.2011. Determination of weight of internal and observation of behavior in SPRN transgenic mice[J]. Chinese Journal of Veterinary, 31(4): 614-618.) [11] 王海丽, 张福宏, 宛博. 2021. 一种六月龄湖羊体重的测量和预测模型[P]. 中国, CN112733084A. 2021-04-30. (Wang H L, Zhang F H, Wan B. 2021. A body weight measurement and prediction model for six-month-old Hu sheep[P]. China, CN112733084A. 2021-04-30.) [12] 王静, 马玉宝. 2018. 胰高血糖素样肽1/胰高血糖素受体双重激动剂治疗肥胖症研究进展[J]. 中华保健医学杂志, 20(01): 81-83. (Wang J, Ma Y B.2018. Research progress of glucagon-like peptide 1/glucagon receptor dual agonist in the treatment of obesity[J]. Chinese Journal of Health and Medicine, 20(01): 81-83.) [13] 王伟, 张圆, 吴利锋, 等. 2020. 湖羊饲养及繁育管理要点[J]. 中国畜禽种业, 16(11): 98-99. (Wang W, Zhang Y, Wu L F, et al.2020. Feeding and breeding management of hu sheep[J]. China Livestock and Poultry Seed Industry, 16(11): 98-99.) [14] 恽时锋, 胡玉红, 田小芸. 2004. 不同品种实验兔主要脏器重量及脏器系数的研究[J]. 中国比较医学杂志, (06): 34-38. (Yun S F, Hu Y H, Tian X Y. 2004. Study on the chief organ weight and coefficients of different spieces of experimental rabbits[J]. Chinese Journal of Comparative Medicine, (06): 34-38.) [15] 张晋青, 岳度兵, 罗海玲, 等. 2010. 日粮中维生素E水平对敖汉细毛羊内脏器官生长发育的影响[J]. 中国畜牧杂志, 46(17): 43-46. (Zhang J Q, Yue D B, Luo H L, et al.2010. Effects of dietary vitamin E level on growth and development of visceral organs in aohan fine wool sheep[J]. Chinese Journal of Animal Science, 46(17): 43-46.) [16] Bahrami J, Longuet C, Baggio L L, et al.2010. Glucagon-like peptide-2 receptor modulates islet adaptation to metabolic stress in the ob/ob mouse[J]. Gastroenterology, 139(3): 857-868. [17] Christensen M, Knop F K, Vilsbll T, et al.2010. Glucagon-like peptide-2, but not glucose-dependent insulinotropic polypeptide, stimulates glucagon release in patients with type 1 diabetes[J]. Regulatory Peptides, 163(1-3): 96-101. [18] Drucker D J, Yusta B.2013. Physiology and pharmacology of the enteroendocrine hormone glucagon-like peptide-2[J]. Annual Review of Physiology, 76(1): 561-583. [19] Fuchs S, Yusta B, Baggio L L, et al.2020. Loss of Glp2r signaling activates hepatic stellate cells and exacerbates diet-induced steatohepatitis in mice[J]. Journal of Clinical Insight, 5(8): 1-8. [20] Gökdal O, Atay O, Eren V, et al.2012. Fattening performance, carcass and meat quality characteristics of Kivircik male lambs[J]. Tropical Animal Health and Production, 44(7): 1491-1496. [21] Guan X.2014. The CNS glucagon-like peptide-2 receptor in the control of energy balance and glucose homeostasis[J]. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 307(6): R585. [22] He C, Holme J, Anthony J.2014. SNP genotyping: The KASP assay[J]. Methods in Molecular Biology, 1145: 75-86. [23] Kushanov F N, Turaev O S, Ernazarova D K, et al.2021. Genetic diversity, QTL mapping, and marker-assisted selection technology in cotton (Gossypium spp.)[J]. Frontiers in Plant Science, 12: 2971-2988. [24] Lee S J, Lee J, Li K K, et al.2012. Disruption of the murine Glp2r impairs paneth cell function and increases susceptibility to small bowel enteritis[J]. Endocrinology, 153(3): 1141-1151. [25] Li F, Lu L, Peng Y, et al.2017. Increased GLP2R expression in gastric chief cells of patients with severe obesity regardless of diabetes status[J]. International journal of obesity (Lond), 41(8): 1303-1305. [26] Lutz T A, Osto E.2016. Glucagon-like peptide-1, glucagon-like peptide-2, and lipid metabolism[J]. Current opinion in lipidology, 27(3): 257-263. [27] Mo C, Zhong Y, Wang Y, et al.2014. Characterization of glucagon-like peptide 2 receptor (GLP2R) gene in chickens: Functional analysis, tissue distribution, and developmental expression profile of GLP2R in embryonic intestine[J]. Domestic Animal Endocrinology, 48: 1-6. [28] Park C R, Jin M M, Park S, et al.2013. A novel glucagon-related peptide (GCRP) and its receptor GCRPR account for coevolution of their family members in vertebrates[J]. PLOS ONE, 8(6): e65420. [29] Sun W, Chen L N, Zhou Q, et al.2020. A unique hormonal recognition feature of the human glucagon-like peptide-2 receptor[J]. Cell Research. 30(12): 1098-1108. [30] Taher J, Baker C, Alvares D, et al.2018. GLP-2 dysregulates hepatic lipoprotein metabolism inducing fatty liver and VLDL overproduction in male hamsters and mice[J]. Endocrinology, 159(9): 3340-3350. [31] Yusta B, Estall J, Drucker D J.2002. Glucagon-like peptide-2 receptor activation engages bad and glycogen synthase kinase-3 in a protein kinase A-dependent manner and prevents apoptosis following inhibition of phosphatidylinositol 3-kinase[J]. The Journal of biological chemistry, 277(28): 24896-24906.