Genome-wide Identification and Expression Analysis of bZIP Family Genes in Botrytis cinerea
LI Bai1,2,3, ZHANG Zi-Yan1, ZHANG Qiang1,2, CAO Hong-Zhe1,2, ZANG Jin-Ping1,2, ZHANG Kang1,2,3, XING Ji-Hong1,2,3,*, DONG Jin-Gao2,3,4,*
1 College of Life Sciences, Hebei Agricultural University, Baoding 071000, China; 2 Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Baoding 071000, China; 3 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071000, China; 4 College of Plant Protection, Hebei Agricultural University, Baoding 071000, China
Abstract：The bZIP (basic leucine zipper, bZIP) transcription factors are widely present in phytopathogenic fungi, and play an important regulatory role in the morphogenesis and infection of pathogens. Systematic studies of the bZIP transcription factor family in Botrytis cinerea have been rarely reported. In this study, the bZIP family genes in Botrytis cinerea were genome-wide identified, the protein physicochemical properties, phylogenetic evolution, conserved domain, and the expression pattern in pathogen conidia during development and infection period were analyzed. Meanwhile, qPCR was used to detect the expression of bZIP family genes after NaCl and H2O2 treatment. The results showed that there were 16 bZIP family genes identified from Botrytis cinerea genome, which were divided into 4 subfamilies by phylogenetic analysis. All bZIP family genes of Botrytis cinerea contained the typical BRLZ domain of bZIP family. Some genes showed high expression levels at different development of conidia and infection stages of Botrytis cinerea. All bZIP family genes of Botrytis cinerea were significantly down-regulated after NaCl and H2O2 treatment. These results indicated that bZIP family genes of Botrytis cinerea played an important role in the growth and development, infection process and response to salt stress and oxidative stress. This study provides a theoretical basis for further revealing the function and molecular mechanism of bZIP family genes of Botrytis cinerea.
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