Abstract：Pseudorabies virus (PRV) infection causes Pseudorabies (PR), which is an acute contact contagious disease in pigs (Sus scrofa). Vaccines are broadly used to control PR. But immunity failures commonly happen due to the prevalence of PRV variant (PRV typeⅡ). In recent years, cases of PRV infection in humans (Homo sapiens) made PRV a global public health threat. However, the pathogenesis of PRV remains vague. To fully understand the molecular change of host cells caused by PRV infection, the PRV typeⅡstrain PRV-DX was used to infect pig kidney cells PK-15 to explore the differential transcriptome by high throughput sequencing. Results showed that 3 595 genes were up-regulated and 3 604 genes were down-regulated. Twelve differentially expressed genes were randomly selected for qPCR verification, and the changing trend was consistent with that of transcriptome data. GO annotation and KEGG signaling pathway enrichment indicated that the differentially expressed genes mainly focused on the pathways such as metabolism, gene expression and immunity. The present study provides important basic data and research direction for further understanding the pathogenic mechanism of PRV infection.
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