Research Progress of LAMP Amplicons Detection Method and the Application of Gene Editing Technology
BAI Rong, BAI Lin-Lin, WANG Shao-Yun, ZHANG Fang*
Fujian Key Laboratory of Inspection and Quarantine Technology Research, College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China
Abstract：With the rapid development of isothermalamplification technology, the method to detect amplicons has become the key restricting its rapid popularization and application. Given the characteristics of Loop-mediated isothermal amplification(LAMP) reaction and the current amplicons detection methods, we summarized the progresses which have been made in recent years, including methods based on by-product generation analysis, methods aiming for multiplex detection, and the sequence-specific ones with visual readouts (e.g. CRISPR/Cas based ones). The possible opportunities and challenges are also been discussed. This review provides support for the nucleic acid analysis industry and applications based on nucleic acid amplification.
白榕, 白琳琳, 汪少芸, 张芳. LAMP扩增产物检测方法研究进展及基因编辑技术在其中的应用[J]. 农业生物技术学报, 2021, 29(10): 2016-2030.
BAI Rong, BAI Lin-Lin, WANG Shao-Yun, ZHANG Fang. Research Progress of LAMP Amplicons Detection Method and the Application of Gene Editing Technology. 农业生物技术学报, 2021, 29(10): 2016-2030.
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