Cloning, Subcellular Localization and Expression Analysis of MAPK Genes from Vitis yeshanesis
ZHU Zi-Guo1, ZHANG Qing-Tian1, LI Xiu-Jie1, HAN Zhen1, LI Gui-Rong2, LI Bo1, *
1 Shandong Institute of Pomology, Taian 271000, China; 2 College of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China
Abstract：Mitogen-activated protein kinase (MAPK) cascade pathway plays an important role in plant growth and development and signal transduction in response to stress. However less the role of MAPK family members were reported in Chinese wild grape (Vitis sp.). In this study, two novel MAPK genes VyMAPK2 and VyMAPK3 (GenBank No. MK942078, MK942077) were obtained from Vitis yeshanesis. VyMAPK2 and VyMAPK3 proteins belonged to the B and A subgroup of the MAPK family, respectively, which all contained 11 conserved protein kinase subdomains, TEY motifs, activation-loop, common docking domain, P-loop and C-loop. Subcellular localization analysis showed that VyMAPK2 was mainly distributed in the nucleus, VyMAPK3 was mainly distributed in the nucleus and cytoplasm. Gene expression analysis showed that VyMAPK2 was mainly expressed in root and VyMAPK3 was mainly expressed in leaves. Under exogenous hormone treatments, auxin, gibberellin, 6-benzyladenine, ethylene, abscisic acid, salicylic acid, and methyl jasmonate could significantly induced the expression of VyMAPK3, but there was no significant change of the expression of VyMAPK2 (P<0.05). Under stress, drought, high salinity and low temperature could significantly induced the expression of VyMAPK3 gene (P<0.05), while VyMAPK2 was only induced by drought. The above results showed that VyMAPK3 gene played an important role in the development and resistance to external stresses in V. yeshanesis. This study will be helpful to understand the stress resistance mechanism of wild grape in China and supply the genes for breeding.
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