Characterization of the Hsp90 Gene in the White Tip Nematode(Aphelenchoides besseyi) and Its Expression in Response to Environmental Stresses, Early Infection and Feeding
Abstract:Hot shock protein 90 (Hsp90) regulates multitudes of biological processes, such as cell metabolism, normal development and environmental stress tolerance. Herein the full-length cDNA and the corresponding gene of the heat shock protein 90, named Ab-hsp90, were isolated and characterized in the plant parasitic nematode Aphelnchoides besseyi, the agent of the rice white-tip disease. The full-length Ab-hsp90 cDNA contained a 5' UTR of 69, an ORF of 2 139 bp encoding a polypeptide of 712 amino acids and a 3' UTR of 137 bp. The deduced amino acid sequence of Ab-hsp90 showed that high similarity with other known Hsp90s, and the Maximum Likehood tree indicates Ab-hsp90 was located in the clade of plant nematodes. Five conserved amino acid signature indicated that Ab-hsp90 was a cytosolic member of the Hsp90 family. The gene consisted of 9 exons and 8 introns. Expression levels of Ab-hsp90 were determined by quantitative real-time PCR. Ab-hsp90 mRNA was constitutively expressed in all developmental stages of A. besseyi, but the highest transcript abundance existed in egg and the adult nematodes. Exposing the nematode to desiccation condition, relatively low osmotic stress and short-term abamectin solution promote Ab-hsp90 transcription. Ab-hsp90 was also induced in the nematodes under the low temperature (4 ℃) at 1.5 h but continuously expressed in high temperature (40 ℃) in 18 h. Interestingly, differential expression of Ab-hsp90 was detected in A. besseyi infecting the resistant and rice seedlings in 72 h. A burst of expression of Ab-hsp90 was present in the inoculated nematodes on the resistant rice seedlings, while its expression was upregulated in 48 h and then dropped at 72 h in the susceptible rice. Moreover, although the differential expression along with the developmental stages of A. besseyi, the mRNA level of Ab-hsp90 was also largely induced in the nematodes feeding on the callus of Daucus carota and hyphae of Botrytis cinerea in 9 d. Our data suggested that Ab-hsp90 can assist A. besseyi to cope with environmental stresses, infect plants and feeding. The results may provide a new perspective to dissecting the functions of hsp90 proteins in plant parasitic nematodes, which thus is conducive to the further study of nematode and plant interactions.
冯辉 陈曦 束兆林 姚克兵 魏利辉. 水稻干尖线虫Hsp90基因克隆及在不同逆境、侵染早期和取食过程的表达差异[J]. , 2016, 24(11): 1741-1753.
Hui Feng Li-Hui Wei. Characterization of the Hsp90 Gene in the White Tip Nematode(Aphelenchoides besseyi) and Its Expression in Response to Environmental Stresses, Early Infection and Feeding. , 2016, 24(11): 1741-1753.
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