Genetic Diversity of In situ Conserved Dongxiang Wild Rice Detected by SSR
KONG De-li;XIE Jian-kun;ZHUANG Jie-yun;FAN Ye-yang;WAN Yong
Institute of Rice Research, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China ; 2. National Rice Research Institute, Hangzhou 310006, China; 3. Jiangxi Agriculture University, Nanchang 330200,China
Abstract:The genetic diversity analysis is important precondition for germ plasm resource's collection and utilization. The genetic diversity of 113 Dongxiang wild rice(Oryza rufipogon Griff.) was evaluated by 24 pairs of simple sequence repeat primers. A total of 114 alleles were generated with average allele per primer pair of 4.75, indicating that the Dongxiang wild rice had high genetic diversity. Clustering result showed that some individuals in the same colony were similar, the minimal genetic distances of the individuals in two colonies were 0 and in the other one was 0.018, the other individuals in the same colony were highly variant and the maximal genetic distance was 0.851. Genetic distance of intrinsic-populations were 0.171~0.435, being smaller than the values of inter-population which were 0.432~0.652. Consequently, the present populations of Dongxiang wild rice must be preserved strictly in original place.