The Genetic Effect Analysis of the Major QTL qTkw-2D for Grain Weight in Wheat (Triticum aestivum)
ZHANG Na1, ZHANG Shuai2, ZHI Li-Ya2, SONG Li-Qiang2, LIU Dong-Tao1, MA Hong-Bo1, JI Jun2,*
1 Jiangsu Xuhuai Regional Institute of Agricultural Sciences, Xuzhou 221131, China; 2 Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang 050021, China
Abstract：In wheat (Triticum aestivum), grain weight selecting is effective for improvement of grain yield as its high heredity and stable phenotype. In previous study, a stable QTL for grain weight, qTkw-2D, was identified and this QTL has been verified in several populations. In this study, the linkage marker Xcfd233 and Wheat660K SNP array were used to scan 3 000 high generation of breeding population. Finally, near isogenic lines (NILs) of qTkw-2D were achieved in the F6 generation of 'Kenong 2011'. By comparison the pericarp cells size of the grain slices from two NILs, it was confirmed that qTkw-2D mainly affected grain width by affecting the size of inner pericarp cells. The analysis results of filling rate showed that qTkw-2D mainly affected grain weight by affecting the grain filling rate during middle and late filling stage. The grain weight in different environments at individual and population level of NILs indicated that qTkw-2D influenced grain yield in both levels and haplotype A was of great importance to maintain grain yield during poor harvest environments. This study provides reference for grain weight improvement and function gene cloning.
张娜, 张帅, 支丽雅, 宋利强, 刘东涛, 马红勃, 纪军. 小麦粒重关键数量性状位点qTkw-2D遗传效应分析[J]. 农业生物技术学报, 2023, 31(10): 1999-2006.
ZHANG Na, ZHANG Shuai, ZHI Li-Ya, SONG Li-Qiang, LIU Dong-Tao, MA Hong-Bo, JI Jun. The Genetic Effect Analysis of the Major QTL qTkw-2D for Grain Weight in Wheat (Triticum aestivum). 农业生物技术学报, 2023, 31(10): 1999-2006.
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