Abstract：As the direct receptor of abscisic acid (ABA), Pyrabactin resistance 1-like (PYL) protein plays an important role in plant growth, development and stress. In order to explore the function of PYL gene family in strawberry, this study identified PYL gene family members in woodland strawberry (Fragaria vesca) and pineapple strawberry (F. ananassa) using bioinformatic methods, and analyzed the expression of PYL gene under different simulated stress conditions by qPCR. The results showed that there were 16 FvPYL genes distributed on 7 chromosomes in woodland strawberry and 40 FaPYL genes distributed on 18 chromosomes in pineapple strawberry. The gene size span of FaPYL gene family was larger than that of FvPYL. Their protein structure was similar and mainly contained α-helix and random coil. In addition, except FvPYL15 and FaPYL40, the other proteins were hydrophilic and mainly expressed in chloroplast, cytoplasm and nucleus. Phylogenetic analysis showed that FaPYL and FvPYL genes were distributed in 5 subfamilies, and the gene structure and motif position of the same species in the same subtribe were basically the same. There were only 3 pairs of segmental duplications in FvPYL genes, but a large number of segmental duplications and some tandem repeats in FaPYL genes. Selection pressure analysis of genes found that strawberry PYL genes were biased toward purification selection. Cis-acting elements analysis found that PYL family genes mainly contained elements related to light, hormone, stress, growth and development. As detected by qPCR, the relative expression levels of strawberry PYL genes were significantly different under different treatments. Under ABA treatment, FaPYL and FvPYL were up-regulated except FvPYL8. Under NaCl treatment, most FaPYL genes were up-regulated to varying degrees, among which FaPYL21 and FaPYL40 were strongly induced by 200 μmol/L NaCl. FvPYL genes expression were overall upregulated, with the relative expression of FvPYL11 was 60 times higher than that of the control. Under PEG treatment, FvPYL genes were positively regulated, FvPYL1 and FvPYL7 were significantly up-regulated. Compared with woodland strawberry, the upregulation range of FaPYL genes were significantly different, among which the relative up-regulation levels of FaPYL2 and FaPYL7 were extremely significant. This study provides a theoretical basis for functional mining and application research of PYL gene family in strawberry.
郭丽丽, 卢世雄, 乃国洁, 马维峰, 毛娟. 草莓PYL基因家族的鉴定与非生物胁迫下的表达分析[J]. 农业生物技术学报, 2022, 30(12): 2315-2332.
GUO Li-Li, LU Shi-Xiong, NAI Guo-Jie, MA Wei-Feng, MAO Juan. Identification of PYL Gene Family and Expression Analysis Under Abiotic Stress in Strawberry. 农业生物技术学报, 2022, 30(12): 2315-2332.
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