Tissue Expression of Lnc_000588 in Sunite Sheep (Ovis aries) and Prediction of Its Interaction Molecules in Regulating Muscle Growth and Development
GONG Wen-Dian1, WANG Rui-Xue1, WANG Rong1, LIU Jun-Yang1, MU Qing1, Terigele1, WU Zi-Xian2, LI Yun-Hua2, LIU Jia-Sen2,*, ZHAO Yan-Hong1,*
1 College of Animal Science, Inner Mongolia Agricultural University/Inner Mongolia Key Laboratory of Animal Genetics, Breeding and Reproduction/Key Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Hohhot 010018, China; 2 Animal Husbandry Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China
Abstract：Sunite sheep (Ovis aries) is high-quality mutton sheep resources in China and one of the main sources of mutton in northern China. Long non-coding RNA (LncRNA) plays an important role in muscle development in O. aries. To predict the regulatory network of Lnc_000588 in skeletal muscle development of Sunite sheep, in this study, the real-time quantitative PCR analysis was performed on its tissue expression in different parts, and the prediction of bioinformatics interaction of targeted miRNA-mRNA was carried out. Meanwhile, the expression of Lnc_000588 was detected in mouse (Mus musculus) C2C12 cells. Lnc_000588 was expressed in all tissues, and the expression level in the longissimus dorsi muscle was significantly higher than that in other tissues (P<0.01); there was no significant difference in Lnc_000588 during cell proliferation, and the expression level in C2C12 cells increased with the prolongation of differentiation time and the increase of myotubes, the expression after 48 h was extremely significantly higher than that in the earlier period (P<0.01). From Lnc_000588 involved in the regulation of the muscle growth and development through miRNA, a total of 32 miRNAs were predicted, of which 3 miRNAs were found to be related with muscle development. The target genes of these 3 miRNAs are enriched in the Ras signaling pathway (oas04014: Ras 66 pathways including), Rap1 signaling pathway (oas04015: Rap1 signaling pathway) and AMPK signaling pathway (oas04152: AMPK signaling pathway). In conclusion, the expression level of Lnc_000588 in Sunite sheep muscle tissue was significantly higher than that in other tissues, which may be involved in the differentiation of C2C12 cells. This study provides basic data for exploring the regulatory function of LncRNA involved in the muscle tissue of Sunite sheep.
宫文典, 王瑞雪, 王荣, 刘俊阳, 穆卿, 特日格勒, 吴子贤, 李蕴华, 刘佳森, 赵艳红. 拟苏尼特羊Lnc_000588的组织表达及其在调控肌肉生长发育中互作分子的预测[J]. 农业生物技术学报, 2022, 30(12): 2333-2343.
GONG Wen-Dian, WANG Rui-Xue, WANG Rong, LIU Jun-Yang, MU Qing, Terigele, WU Zi-Xian, LI Yun-Hua, LIU Jia-Sen, ZHAO Yan-Hong. Tissue Expression of Lnc_000588 in Sunite Sheep (Ovis aries) and Prediction of Its Interaction Molecules in Regulating Muscle Growth and Development. 农业生物技术学报, 2022, 30(12): 2333-2343.
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