Expression Pattern of Inosine Monophosphate Specific Deposition Related Genes in Muscle Tissue of Jingyuan Chicken (Gallus gallus) and Pudong Chicken
ZHANG Juan1, YU Bao-Jun1, ZHU Ji-Hong2, HU Hong-Hong1, WANG Wei-Zhen1, CAI Zheng-Yun1, GU Ya-Ling1, XIN Guo-Sheng3,*
1 College of Agriculture, Ningxia University, Yinchuan 750021, China; 2 Ningxia Animal Husbandry Workstation, Yinchuan 750021, China; 3 College of Life Sciences, Ningxia University/Ningxia Feed Engineering Technology Research Center, Yinchuan 750021, China
Abstract：Inosine monophosphate (IMP), as an important material basis of meat flavor, has become an important index to evaluate the umami taste and freshness of meat. In order to explore the role of key regulatory genes in the process of inosine monophosphate deposition in muscle tissue of Jingyuan chicken (Gallus gallus) and Pudong chicken, and to provide theoretical basis for the study of muscle quality among breeds, in this study, based on IMP, inosine, and some physical indexes of Jingyuan chicken and Pudong chicken, the differentially expressed genes related to meat quality among different breeds were screened by transcriptome sequencing, and the expression differences of cyclin A1 interacting protein (protein interacting with cyclin A1, PROCA1) and chicken bile toxin 1 (axis inhibition protein 1, AXIN1) gene in breast muscle tissues of Jingyuan chicken and Pudong chicken were detected by the qPCR method. The results showed that the IMP content of Jingyuan chicken was higher than that of Pudong chicken, and the IMP content of breast muscle of Jingyuan chicken and Pudong chicken was significantly higher than that of leg muscle (P<0.05). The results of transcriptome sequencing showed that the differential genes PROCA1 and AXIN1 among different varieties were screened in glycerol phospholipid metabolism pathway, arachidonic acid metabolism, and Wnt signal pathway. By comparing the gene expression of different breeds, it was found that the expression of PROCA1 and AXIN1 genes in breast muscle of Jingyuan chicken was higher than that of Pudong chicken, and the trend of differential expression was consistent with that of transcriptome sequencing. In summary, PROCA1 and AXIN1 genes may be candidate genes that affect the specific deposition of inosinic acid in the muscle tissue of Jingyuan chicken and Pudong chicken. The results of this study provide a reference for further exploring the regulation mechanism of IMP-specific deposition of PROCA1 and AXIN1 genes.
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