Correlation Analysis Between Heterosis Performances, Gene Expression at Seeding Stage and Yield of Hybrids Rice (Oryza sativa)
WANG Ying-Heng1,2, Xu Jing3, CAI Qiu-Hua1,2, LIN Qiang1,2, HE Wei1,2, XIE Hong-Guang1,2, XIE Hua-An1,2, ZHANG Jian-Fu1,2,*
1 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, China; 2 Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural, P. R. China/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology/Base of South-China, National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China, Fuzhou 350003, China; 3 Institute of Cereal Crops, Hainan Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Breeding of Hainan Province, Haikou 571100 ,China
摘要植物在发育早期就呈现出明显的杂种优势,苗期是水稻(Oryza sativa)株型形态建成和产量形成的重要时期。为了解苗期杂种优势及基因表达与产量杂种优势的相关性、探索通过水稻苗期表现预测杂交稻产量潜力的可能性,本研究选取杂交水稻有代表性亲本18份,配制45个不完全双列杂交组合,系统考查杂交组合苗期根和苗的杂种优势、基因表达及其与单株产量之间的关系。研究结果表明,同一时期根和苗的性状间、同一组织不同时期性状间都有相关性,苗期性状间有12对性状相关性显著(P<0.05),有2个苗期性状与田间单株产量呈正相关(P<0.05);杂交稻组合在萌发早期(发芽第3天, 3 d after sowing, 3DAS)杂种优势最高;6个苗期性状的中亲优势(middle parent heterosis, MPH)有13对性状正相关(P<0.01),根长-3DAS,根长-14DAS,株高-3DAS的MPH和单株产量MPH正相关(P<0.05);分析了4个根系发育相关基因在杂交稻组合和亲本间播种后第17天的表达模式,有3个基因与第17天植株的根干重和苗干重相关,4个基因的表达水平均与田间单株产量呈显著相关(P<0.05)。杂交水稻组合在种子萌发早期就表现出极强的杂种优势,苗期根和苗各性状间的杂种优势普遍具有相关性。部分性状的杂种优势以及与性状相关的基因表达水平与成熟期单株产量显著相关。本研究为利用水稻早期性状预测产量杂种优势模型的建立和杂交水稻新品种选育提供了理论基础。
Abstract：Plants often show significant heterosis at the early developmental stage, and the seedling stage is the most important stage for rice (Oryza sativa) architectural development and yielding ability. In order to investigate the relationship between heterosis performance as well as genes expression levels of hybrid rice at seedling stage and the heterosis of yield in field, and to explore the possibility of yield potential prediction for hybrid rice with their performance at seedling stage, 18 representative hybrid parental lines were obtained to make an incomplete diallel crosses, and 45 hybrids rice were obtained. Heterosis performance of root, shoot, and genes expression pattern at the seedling stage, as well as the relationship between yield and traits mentioned were systematically analyzed. The correlated relationship was detected between the above-ground and underground traits at the same seedling stage, as well as traits in two different periods for the same tissue. 12 pairs of comparison between the trait value were significantly correlated, and two seedling traits were positively correlated with yield (P<0.05). Middle parent heterosis (MPH) heterosis at the early stage of seedling (3DAS) was higher than any other stages. 13 pairs of comparison between MPH values of seedling traits were positively correlated. Root length (RL)-3DAS, RL-14DAS and plant height (PH)-3DAS were correlated with the MPH value of yield (P<0.05). In addition, the expression patterns of the 4 root development related genes were observed at 17 DAS among hybrids and parental lines. Three of them were correlated with the dry root weight and shoot at 17 DAS, and all of them were correlated with the yield in the field (P<0.05). High level of heterosis performances were detected at early seedling stage. The correlations of heterosis value among traits at seedling stage were ubiquitous. Some seedling traits and the expression level of root related genes in hybrids at seedling stage were significantly correlated with the yield of hybrids. This study shed light on the model for heterosis prediction based on the early traits of hybrid rice and also provides a theoretical basis for hybrid rice breeding.
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