Polymorphism of Gene Coding Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep (Ovis aries) Varieties
SUN Wei-Bo1,2, LIU Li-Shan1, ZHANG Li-Ping2, WANG Cai-Lian1, LANG Xia1,*
1 Institute of Animal & Pasture Science and Green Agriculture, Gansu Academy of Agricultural Science / Key Laboratory for Sheep, Goat, and Cattle Germplasm and Straw Feed in Gansu Province, Lanzhou 730070, China; 2 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：In order to explore the genetic effect of polymorphism in the bone morphogenetic protein receptor-IB (BMPR-IB) gene coding region on the reproductive performance of sheep (Ovis aries), ewes of 4 sheep breeds were used as the research object in this study, which includes 65 multiple Small Tail Han sheep (SM), 71 twins Mongolian sheep (MT), 70 singleton Mongolian sheep (MS), 52 twins Oula sheep (ZT), 51 singletons Oula sheep (ZS), and 74 singletons Dorper sheep (DS). Using PCR combined DNA direct sequencing method, the polymorphism of 597 loci in the coding region of BMPR-IB gene of different fertility ewes was tested, and the correlation analysis was performed with the litter size. The results showed that: G/A mutation was detected at the 597 site of the coding region of the BMPR-IB gene, GG, GA and AA genotypes were detected in each experimental group sheep. The dominant genotype in SM was AA, and the dominant allele was A. The dominant genotype in MT, MS, ZT and ZS was GA, the dominant allele was G. The dominant genotype in DS was GG, and the dominant allele was G. All the experimental group sheep were in moderate polymorphism (0.25<PIC<0.5); the litter size of homozygous mutation AA type was significantly higher than that wild GG type and heterozygous mutant GA type in the Oula sheep experimental population (P<0.05). Therefore, this locus can be used as a potential molecular genetic marker site for sheep lambing number. This study provides theoretical support for the genetic mechanism and breeding of sheep lambing traits.
孙渭博, 刘立山, 张利平, 王彩莲, 郎侠. 四种绵羊BMPR-IB基因编码区多态性及其与胎产羔数的相关分析[J]. 农业生物技术学报, 2020, 28(12): 2189-2199.
SUN Wei-Bo, LIU Li-Shan, ZHANG Li-Ping, WANG Cai-Lian, LANG Xia. Polymorphism of Gene Coding Region of BMPR-IB Gene and Its Correlation with Litter Size in Four Sheep (Ovis aries) Varieties. 农业生物技术学报, 2020, 28(12): 2189-2199.
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