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2025年4月3日 星期四
农业生物技术学报  2019, Vol. 27 Issue (8): 1341-1350    DOI: 10.3969/j.issn.1674-7968.2019.08.002
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
梨钾通道基因PbAKT1的克隆及其表达分析
杨晗1, 李岩1, 申长卫2, 金雨濛1, 石晓倩1, 谢昶琰1, 梅新兰1, 徐阳春1, 董彩霞1,*
1 南京农业大学 资源与环境学院/江苏省固体有机废弃物资源化研究重点实验室/江苏省有机固体废弃物协同创新中心/教育部资源节约型肥料工程技术研究中心,南京 210095;
2 河南科技学院 资源与环境学院,新乡 453003
Cloning and Expression Analysis of Potassium Channel Gene PbAKT1 in Pear (Pyrus betulifolia)
YANG Han1, LI Yan1, SHEN Chang-Wei2, JIN Yu-Meng1, SHI Xiao-Qian1, XIE Chang-Yan1, Mei Xin-Lan1, XU Yang-Chun1, DONG Cai-Xia1,*
1 Jiangsu Provincial Key Lab for Organic Solid Waste Utilization/ National Engineering Research Center for Organic-based Fertilizers/ Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/ College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing,210095,China;
2 School of Resources and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China
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摘要 AKT1 (Arabidopsis potassium transport 1)是重要的钾通道蛋白基因,参与植物钾吸收和转运过程。为了探究梨(Pyrus betulifolia) PbAKT1基因对钾的响应及表达模式,本研究采用不同浓度K+处理杜梨幼苗,对PbAKT1基因进行克隆并对其所编码的蛋白进行组织特异性分析。结果表明,克隆获得的PbAKT1基因(GenBank No. MN150549) cDNA全长2 646 bp,编码881个氨基酸,有Shaker家族典型的S1~S6的跨膜结构和1个loop环;氨基酸序列的同源关系分析表明,梨PbAKT1基因与苹果(Malus domestica) MdAKT1基因的亲缘关系最近;亚细胞定位发现,该基因主要定位于细胞膜上,为亲水性稳定蛋白;PbAKT1基因在梨苗根、茎、叶片中都有表达,不存在组织特异性;缺钾条件下,该基因有明显响应。综上所述,梨PbAKT1的表达受到缺钾胁迫的诱导,可能参与梨树K+吸收和转运过程。本研究结果为进一步揭示梨树耐低钾分子机制提供基础了资料。
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作者相关文章
杨晗
李岩
申长卫
金雨濛
石晓倩
谢昶琰
梅新兰
徐阳春
董彩霞
关键词 钾通道蛋白基因表达PbAKT1亚细胞定位    
AbstractAKT1 (Arabidopsis potassium transport 1) is an important potassium channel protein gene involved in plant potassium absorption and transport. In order to study the response and expression pattern of PbAKT1 gene in Pyrus betulifolia to potassium, this study adopted K+ at different concentrations (0.1 mmol/L and 3 mmol/L K+) to treat pear seedlings, cloned PbAKT1 gene and analyzed the tissue specificity of its encoded protein. Results showed that the PbAKT1 gene (GenBank No. MN150549) cDNA was 2 646 bp in length, encoding 881 amino acids. The gene had a transmembrane structure with a typical S1~S6 of the Shaker family and a loop ring. The homology analysis of amino acid sequence indicated that there was a close phylogenetic relationship between PbAKT1 gene and MdAKT1 gene (Malus domestica). Subcellular localization showed that the gene mainly presented in the cell membrane and was a hydrophilic stable protein. PbAKT1 gene was found no tissue specificity in roots, stems and leaves of pear seedlings and its expression obviously responded to low potassium stress. It was indicated that the expression of PbAKT1 was induced by potassium supply and might be involved in K+ absorption and transport of pear trees. It provides basic information for further research of the molecular mechanism to low potassium stress in pear.
Key wordsPear    Potassium channel gene    Gene expression    PbAKT1    Subcellular localization
收稿日期: 2019-01-31     
ZTFLH:  S661.2  
基金资助:国家自然科学基金(No.31872172)和国家现代农业产业技术体系建设专项资金项目(CARS-28-10)
通讯作者: *,cxdong@njau.edu.cn   
引用本文:   
杨晗, 李岩, 申长卫, 金雨濛, 石晓倩, 谢昶琰, 梅新兰, 徐阳春, 董彩霞. 梨钾通道基因PbAKT1的克隆及其表达分析[J]. 农业生物技术学报, 2019, 27(8): 1341-1350.
YANG Han, LI Yan, SHEN Chang-Wei, JIN Yu-Meng, SHI Xiao-Qian, XIE Chang-Yan, Mei Xin-Lan, XU Yang-Chun, DONG Cai-Xia. Cloning and Expression Analysis of Potassium Channel Gene PbAKT1 in Pear (Pyrus betulifolia). 农业生物技术学报, 2019, 27(8): 1341-1350.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.08.002     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I8/1341
 
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