HMOX1 Gene Expression and Assiciation Between Its Polymorphism and Hypoxia Adaptation in Tibetan Sheep (Ovis aries)
HE Jian-Wen1,2, ZHANG Wei1, LIU Xiu1,*, LI Shao-Bin1, WANG Ji-Qing1, HU Jiang1, LUO Yu-Zhu1,*
1 College of Animal Science and Technology/Gansu Key Laboratory of Herbivorous Animal Biotechnology/Gansu Engineering Laboratory of Genetic Improvement in Ruminants, Gansu Agricultural University, Lanzhou 730070, China; 2 GanSu Agriculture Technology College, Lanzhou 730020, China
Abstract：Heme oxygenase 1 (HMOX1) is a stress-inducible enzyme of the heme oxygenase family. HMOX1 plays an important role in the metabolic regulation of hypoxia stress in the body. Tibetan sheep (Ovis aries)have developed a unique hypoxic adaptation strategy during long-term evolution to maintain their own oxygen balance. In order to investigate the relationship between HMOX1 expression and Exon3 polymorphism and hypoxia adaptation in Tibetan sheep, look for molecular markers relating to hypoxia adaptation, this study used Tibetan sheep and Hu sheep (control group) at different altitudes, the expression of tissues and the genotype polymorphisms in exon3 of HMOX1 gene were detected by qRT-PCR and PCR-single-strand conformation polymorphism (PCR-SSCP), and the associations of different genotypes and alleles with blood parameters of sheep were analyzed. Sexuality and the correlation between different genotypes of single polymorphic loci and blood parameters of sheep were analyzed. The results showed that HMOX1 was expressed in tissues of Tibetan sheep and Hu sheep. In Tibet sheep, the gene was highly expressed in spleen (P<0.01), followed by liver, kidney and longissimus dorsi muscle (P<0.05). In Hu sheep, the gene was highly expressed in lung and fat (P<0.01), followed by liver, kidney and spleen (P<0.05). The expressions of HMOX1 gene in lung and fat tissue were significantly different between Tibetan sheep and Hu sheep (P<0.01). Four SNPs loci and 3 alleles of A, B and C were detected in the Exon3 region of HMOX1 gene. By comparing different genotypes between the two varieties, the hemoglobin (HGB) and hematokrit (HCT) values of AB Hu sheep were significantly lower than those of Tibetan sheep (P<0.01). The HGB and HCT values of BB individuals were significantly higher than those of AB (P<0.01), and the AC-type SO2 values were significantly higher than those of AA and BB individuals and c.3775G>A sites. The SO2, HGB and HCT values of MM genotypes in c.3775G>A locus were significantly higher than those of NN genotypes (P<0.05), which indicated that the C.3775G>A locus G of AA, BB and AC genotypes was more favorable for adaptation to hypoxic environment. Therefore, HMOX1 gene can be used as candidate gene.These results provide basic data for exploring the molecular mechanism of hypoxia adaptation in Tibetan sheep.
何建文, 张伟, 刘秀, 李少斌, 王继卿, 胡江, 罗玉柱. 藏绵羊HMOX1基因表达及其多态性与低氧适应性的关联分析[J]. 农业生物技术学报, 2019, 27(3): 441-448.
HE Jian-Wen, ZHANG Wei, LIU Xiu, LI Shao-Bin, WANG Ji-Qing, HU Jiang, LUO Yu-Zhu. HMOX1 Gene Expression and Assiciation Between Its Polymorphism and Hypoxia Adaptation in Tibetan Sheep (Ovis aries). 农业生物技术学报, 2019, 27(3): 441-448.
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