Cloning and Expression Characteristics Analysis of CaERF109 and CaABR1 Gene in Canarium album
LAI Rui-Lian1,*, CHEN Jin1,*, FENG Xin1, WANG Zhong2, CHEN Yi-Ting1, WU Ru-Jian1, **
1 Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2 College of Food and Bioengineering, Hezhou University, Hezhou 542899, China
Abstract：Canarium album is one of the characteristic and precious fruit in tropical and subtropical regions of China. Generally, C. album is poor in cold resistance, thus, often suffers from low temperature freezing damage. Recently, accumulated evidences have revealed that APETALA2/ ethylene responsive factor (AP2/ERF) is closely related to growth and stress response of many plants. To investigate the regulatory functions of AP2/ERF transcription factors in C. album, two AP2/ERF gene superfamily members, named CaERF109 (GenBank No. MH670905) and CaABR1 (abscisic acid repressor 1, GenBank No. MH670906) were cloned from C album cv. Fulan-1 tree, and their bioinformatics and qRT-PCR expression patterns were performed in this study. The results showed that the ORF of CaERF109 and CaABR1 were 888 bp and 1 473 bp respectively, and predicted to encode 295 and 490 amino acids. Bioinformatics analysis showed that the codon biases level of CaERF109 and CaABR1 were low, and both of them encoded unstable hydrophobic basic proteins, contained ERF typical features that belonged to AP2/ERF transcription factor superfamily. Subcellular location prediction showed that CaERF109 and CaABR1 located in mitochondrial matrix and peroxisomes respectively. According to qRT-PCR analysis, both CaERF109 and CaABR1 showed organ-specific expression patterns, and of which was highly expressed in root and leaf respectively. In addition, both genes were extremely significantly up-regulated with the decrease of temperature during cold stress (P<0.01). This research revealed that both CaERF109 and CaABR1 may play different regulation functions during development of organs, and involve in low temperature stress responses of C. album. This study could provide the theoretical foundation for molecular mechanism research of low temperature stress and cold resistance breeding in C. album.
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