Abstract：Prunus mume, a traditional famous flower in China, holds significant ornamental value. GATA transcription factors play a crucial role in the regulation of plant growth and development as well as in the response to abiotic stress. In this study, a genome-wide identification of the GATA gene family in P. mume was conducted, and their expression patterns following cold treatment were systematically analyzed, with the functional characterization of PmGATA12 being comprehensively investigated. The results indicated that a total of 20 PmGATA genes were identified through whole-genome analysis, distribute across 7 chromosomes. Phylogenetic analysis revealed that PmGATA proteins could be divided into 3 subfamilies. Members of the same subfamily have similar conserved motifs and gene structures. Furthermore, by integrating transcriptome and qRT-PCR expression analysis, 4 differentially expressed PmGATA genes (PmGATA6, PmGATA11, PmGATA12, and PmGATA14) were identified. The genetic transformation of Arabidopsis thaliana with PmGATA12 significantly enhanced the cold tolerance of the transgenic lines. This study provides valuable gene resources for the cold-resistance breeding of P. mume and offers new insights into the molecular mechanisms underlying its cold tolerance.
刘钊良, 张军, 金小军, 金松恒, 董彬. 梅花GATA基因家族鉴定及PmGATA12在耐寒性调控中的作用分析[J]. 农业生物技术学报, 2025, 33(4): 771-781.
LIU Zhao-Liang, ZHANG Jun, JIN Xiao-Jun, JIN Song-Heng, DONG Bin. Identification of the Prunus mume GATA Gene Family and Analysis of the Role of PmGATA12 in Cold Tolerance Regulation. 农业生物技术学报, 2025, 33(4): 771-781.
[1] 段美红, 杨益, 李庆卫. 2020. 电导法结合Logistic方程鉴定嫁接繁殖梅花的抗寒性[J]. 中国园林, 36(S1): 67-70.(Duan M H, Yang Y, Li Q W.2020. Identification of cold resistance based on conductance method and logistic equation in the top-grafting of Prunus mume[J]. Chinese Landscape Architecture, 36(S1): 67-70.) [2] 沈超. 2022. 杨树PdGATA19和PdGATA13在干旱和低氮胁迫下的功能研究[D]. 博士学位论文, 北京林业大学, 导师: 尹伟伦, 夏新莉, pp. 66-78.(Shen C.2022. Functional analyses of poplar PdGATA19 and PdGATA13 in response to low-nitrogen and drought stress[D]. Thesis for Ph.D., Beijing Forestry University, Supervisor: Yin W L, Xia X L, pp. 66-78.) [3] Bert D R, Valya V, Boris P, et al.2010. A novel aux/IAA28 signaling cascade activates GATA23-dependent specification of lateral root founder cell identity[J]. Current Biology, 20(19): 1697-1706. [4] Chen C, Wu Y, Li J, et al.2023. TBtools-II: A ''one for all, all for one'' bioinformatics platform for biological big-data mining[J]. Molecular Plant, 16(11): 1733-1742. [5] Daniel-Vedele F, Caboche M.1993. A tobacco cDNA clone encoding a GATA-1 zinc finger protein homologous to regulators of nitrogen metabolism in fungi[J]. Molecular and General Genetics, 240: 365-373. [6] Dong B, Wang Q Q, Zhou D, et al.2024. Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid[J]. Horticultural Plant Journal, 10(2): 573-585. [7] Feng X, Yu Q, Zeng J, et al.2022. Genome-wide identification and characterization of GATA family genes in wheat[J]. BMC Plant Biology, 22(1): 372. [8] Guo M, Yang F, Zhu L, et al.2024. Loss of cold tolerance is conferred by absence of the WRKY34 promoter fragment during tomato evolution[J]. Nature Communications, 15(1): 6667. [9] Gupta P, Nutan K K, Singla-Pareek S L.et al.2017. Abiotic stresses cause differential regulation of alternative splice forms of GATA transcription factor in rice[J]. Frontiers in Plant Science, 8: 1944. [10] Liu P P, Koizuka N, Martin R C.et al.2005. The BME3 (blue micropylar end 3) GATA zinc finger transcription factor is a positive regulator of Arabidopsis seed germination[J]. The Plant Journal, 44(6): 960-971. [11] Lowry J A, Atchley W R.2000. Molecular evolution of the GATA family of transcription factors: Conservation within the DNA-binding domain[J]. Journal of Molecular Evolution, 50: 103-115. [12] Luo X M, Lin W H, Zhu S, et al.2010. Integration of light-and brassinosteroid-signaling pathways by a GATA transcription factor in Arabidopsis[J]. Developmental Cell, 19(6): 872-883. [13] Manfield I W, Devlin P F, Jen C H, et al.2007. Conservation, convergence, and divergence of light-responsive, circadian-regulated, and tissue-specific expression patterns during evolution of the Arabidopsis GATA gene family[J]. Plant Physiology, 143(2): 941-958. [14] Nutan K K, Singla-Pareek S L, Pareek A.2020. The Saltol QTL-localized transcription factor OsGATA8 plays an important role in stress tolerance and seed development in Arabidopsis and rice[J]. Journal of Experimental Botany, 71(2): 684-698. [15] Omichinski J G, Clore G M, Schaad O, et al.1993. NMR structure of a specific DNA complex of Zn-containing DNA binding domain of GATA-1[J]. Science, 261(5120): 438-446. [16] Peng W, Li W, Song N, et al.2021. Genome-wide characterization, evolution, and expression profile analysis of GATA transcription factors in Brachypodium distachyon[J]. International Journal of Molecular Sciences, 22(4): 2026. [17] Reyes J C, Muro-Pastor M I, Florencio F J.2004. The GATA family of transcription factors in Arabidopsis and rice[J]. Plant Physiology, 134(4): 1718-1732. [18] Richter R, Bastakis E, Schwechheimer C.2013. Cross-repressive interactions between SOC1 and the GATAs GNC and GNL/CGA1 in the control of greening, cold tolerance, and flowering time in Arabidopsis[J]. Plant Physiology, 162(4): 1992-2004. [19] Schwechheimer C, Schröder P M, Blaby-Haas C E.2022. Plant GATA factors: Their biology, phylogeny, and phylogenomics[J]. Annual Review of Plant Biology, 73(1): 123-148. [20] Shikata M, Matsuda Y, Ando K, et al.2004. Characterization of Arabidopsis ZIM, a member of a novel plant‐specific GATA factor gene family[J]. Journal of Experimental Botany, 55(397): 631-639. [21] Teakle G R, Manfield I W, Graham J F, et al.2002. Arabidopsis thaliana GATA factors: Organisation, expression andDNA-binding characteristics[J]. Plant Molecular Biology, 50: 43-56. [22] Tremblay M, Sanchez-Ferras O, Bouchard M.2018. GATA transcription factors in development and disease[J]. Development, 145(20): dev164384. [23] Wei X, Li Y, Zhu X, et al.2023. The GATA transcription factor TaGATA1 recruits demethylase TaELF6‐A1 and enhances seed dormancy in wheat by directly regulating TaABI5[J]. Journal of Integrative Plant Biology, 65(5): 1262-1276. [24] Zhan J, Thakare D, Ma C, et al.2015. RNA sequencing of laser-capture microdissected compartments of the maize kernel identifies regulatory modules associated with endosperm cell differentiation[J]. The Plant Cell, 27(3): 513-531. [25] Zhang C, Hou Y, Hao Q, et al.2015. Genome-wide survey of the soybean GATA transcription factor gene family and expression analysis under low nitrogen stress[J]. PLOS ONE, 10(4): e0125174. [26] Zhang H, Wu T, Li Z, et al.2021. OsGATA16, a GATA transcription factor, confers cold tolerance by repressing OsWRKY45-1 at the seedling stage in rice[J]. Rice, 14(1): 42. [27] Zhang X, Ma J, Yang S, et al.2023. Analysis of GATA transcription factors and their expression patterns under abiotic stress in grapevine (Vitis vinifera L.)[J]. BMC Plant Biology, 23(1): 611. [28] Zhang Z, Ren C, Zou L, et al.2018. Characterization of the GATA gene family in Vitis vinifera: Genome-wide analysis, expression profiles, and involvement in light and phytohormone response[J]. Genome, 61(10): 713-723. [29] Zhao T, Wu T, Pei T, et al.2021. Overexpression of SlGATA17 promotes drought tolerance in transgenic tomato plants by enhancing activation of the phenylpropanoid biosynthetic pathway[J]. Frontiers in plant science, 12: 634888. [30] Zhu W, Guo Y, Chen Y, et al.2020. Genome-wide identification, phylogenetic and expression pattern analysis of GATA family genes in Brassica napus[J]. BMC Plant Biology, 20: 1-12.
