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33
2025年8月1日 星期五
农业生物技术学报  2025, Vol. 33 Issue (4): 782-791    DOI: 10.3969/j.issn.1674-7968.2025.04.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
PLAG1基因过表达对3T3-L1前脂肪细胞增殖分化的影响
吴姿仪, 白利瑶, 吴嘉浩, 窦腾飞, 李新建, 韩雪蕾, 乔瑞敏, 王克君, 杨峰, 李秀领*
河南农业大学 动物科技学院, 郑州 450046
Effects of Overexpression of Porcine (Sus Scrofa) PLAG1 Gene on Proliferation and Differentiation of 3T3-L1 Preadipocytes
WU Zi-Yi, BAI Li-Yao, WU Jia-Hao, DOU Teng-Fei, LI Xin-Jian, HAN Xue-Lei, QIAO Rui-Min, WANG Ke-Jun, YANG Feng, LI Xiu-Ling*
College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
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摘要 多形性腺瘤基因1 (plemorphic adenoma gene 1, PLAG1)在调控畜禽生长发育中具有重要作用。本课题组前期通过全基因组关联分析(genome-wide association study, GWAS)方法筛选到该基因与猪(Sus scrofa)的背膘厚(backfat thickness, BF)有关,其可能对脂肪细胞有潜在的调控作用。为进一步探究PLAG1基因与脂肪细胞的关系,本实验以3T3-L1前脂肪细胞为研究对象,并对PLAG1基因进行保守性分析,通过qRT-PCR、CCK-8、流式细胞术和油红O染色等技术探究过表达PLAG1基因对脂肪细胞增殖分化的影响。结果表明,PLAG1基因的保守性较高,在不同物种间的相似度为80%~92%;qRT-PCR结果显示,过表达PLAG1基因后,3T3-L1细胞增殖相关基因的表达极显著高于对照组(P<0.01);CCK-8结果表明过表达PLAG1基因后极显著提高了细胞活性(P<0.01);流式细胞术发现过表达PLAG1基因后S期的细胞数显著增加(P<0.05)。油红O染色结果显示,过表达PLAG1基因后显著降低成脂分化相关基因的表达(P<0.05),细胞脂滴数量也极显著降低(P<0.01)。综上,过表达PLAG1基因促进3T3-L1前脂肪细胞增殖,抑制其分化,说明PLAG1基因可能在脂肪细胞增殖和分化过程中发挥作用。本研究为进一步探究猪PLAG1基因调控脂肪沉积分子机制提供理论参考。
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吴姿仪
白利瑶
吴嘉浩
窦腾飞
李新建
韩雪蕾
乔瑞敏
王克君
杨峰
李秀领
关键词 多形性腺瘤基因1 (PLAG1)增殖分化基因功能3T3-L1前脂肪细胞    
Abstract:Pleomorphic adenoma gene 1 (PLAG1) plays a crucial role in regulating the growth and development of livestock and poultry. Our previous study identified that PLAG1 was associated with backfat thickness (BF) in pigs by genome-wide association study (GWAS), suggesting a potential regulatory effect on adipocytes. To further investigate the relationship between PLAG1 gene and adipocytes, 3T3-L1 cells was used as a research model and PLAG1 conservation was analyzed. The effects of PLAG1 gene overexpression on cell proliferation and differentiation were examined using qRT-PCR, CCK-8 assays, flow cytometry, and Oil Red O staining. Results revealed high conservation of the PLAG1 gene, with sequence similarity among different species ranging from 80% to 92%. qRT-PCR analysis showed that, compared with the control group, the expression of proliferation-related genes in 3T3-L1 cells was extremely significantly increased following PLAG1 overexpression (P<0.01). CCK-8 assays showed that PLAG1 overexpression extremely significantly enhanced cell viability (P<0.01). Flow cytometry demonstrated a significant increase in the number of cells in the S phase following PLAG1 overexpression (P<0.05). Furthermore, Oil Red O staining indicated that the expression of adipogenic differentiation-related genes was significantly decreased after PLAG1 overexpression (P<0.05), accompanied by a extremely significant reduction in lipid droplet formation (P<0.01). In summary, PLAG1 overexpression promoted the proliferation of 3T3-L1 cells while inhibiting their differentiation, indicating that PLAG1 gene might play a regulatory role in the proliferation and differentiation of adipocytes. This study provides a theoretical foundation for further investigation of the molecular mechanisms through which the PLAG1 gene regulates fat deposition in pigs.
Key wordsPlemorphic adenoma gene 1 (PLAG1)    Pigs    Proliferation and differentiation    Gene function    3T3-L1 preadipocytes
收稿日期: 2024-08-18     
中图分类号: S828.9+9
基金资助:国家重点研发计划(2021YFD1301201);河南高等学校重点科研项目(23A230012);河南省农业良种联合攻关项目(2022020101)
通讯作者: * xiulingli@henau.edu.cn   
引用本文:   
吴姿仪, 白利瑶, 吴嘉浩, 窦腾飞, 李新建, 韩雪蕾, 乔瑞敏, 王克君, 杨峰, 李秀领. 猪PLAG1基因过表达对3T3-L1前脂肪细胞增殖分化的影响[J]. 农业生物技术学报, 2025, 33(4): 782-791.
WU Zi-Yi, BAI Li-Yao, WU Jia-Hao, DOU Teng-Fei, LI Xin-Jian, HAN Xue-Lei, QIAO Rui-Min, WANG Ke-Jun, YANG Feng, LI Xiu-Ling. Effects of Overexpression of Porcine (Sus Scrofa) PLAG1 Gene on Proliferation and Differentiation of 3T3-L1 Preadipocytes. 农业生物技术学报, 2025, 33(4): 782-791.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2025.04.007     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2025/V33/I4/782
 
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