Gene Cloning and Expression Analysis of LoSorNINJAs in Oriental Lily (Lilium hybrid divisionⅦ) 'Sorbonne'
ZHU Yun-Tao1, ZHAO Yi-Ran1, CHEN Ming-Hui2, HU Jun-Shu1, YANG Jie1, LIU Xiao-Lin1, SUN Ting-Ting1, KONG Xiang-Hong1, LUO Yuan-Fang1, NIE Yu-Wei1, GAO Xue1, HE Heng-Bin1,*
1 School of Landscape Architecture/Beijing Key Laboratory of Flower Germplasm Innovation and Molecular Breeding/National Flower Engineering Technology Research Center/Urban and Rural Ecological Environment Beijing Laboratory/Ministry of Education Forest and Flower Breeding Laboratory, Beijing Forestry University, Beijing 100083, China; 2 China National Botanical Garden (North Garden),Beijing 100093, China
Abstract：Jasmonic acid (JA) is an important plant endogenous hormone, which plays an important role in plant growth, development and defense response. NINJA (novel interactor of JAZ) protein is an inhibitor of the JA signal transduction pathway, involved in the regulation of plant growth, development, and stress response. In this study, 2 different NINJAs were identified from Oriental lily 'Sorbonne' (Lilium hybrid division Ⅶ cv. 'Sorbonne') based on transcriptome data, and named LoSorNINJA1 (GenBank No. OQ715340) and LoSorNINJA2 (GenBank No. OQ715341), respectively. Bioinformatics analysis showed that both LoSorNINJA1 and LoSorNINJA2 contained conserved EAR motif and Jas domain, which conformed to the characteristics of the NINJA gene family. Phylogenetic analysis indicated that LoSorNINJAs had the closest genetic relationship with the NINJA of Apostasia shenzhenica, Phalaenopsis equestris and Dendrobium catenatum. The results of subcellular localization in tobacco (Nicotiana tabacum) showed that both LoSorNINJA1 and LoSorNINJA2 were located in the nucleus. Expression analysis in different tissues of 'Sorbonne' showed that LoSorNINJA2 was highly expressed in the outer petals and anthers, and LoSorNINJA1 was highly expressed in the upper leaves. The shoot apical of 'Sorbonne' lily bulbs after low-temperature storage and greenhouse transplant were collected and analyzed by qRT-PCR. LoSorNINJA1 had 2 peaks of expression at 2 and 6 w cold storage, while LoSorNINJA2 had only 1 peak of expression at 6 w cold storage. Using GENIE3 to construct a potential gene regulatory network indicated that LoSorNINJA1 might be involved in tissue development and post-embryonic development, and LoSorNINJA2 might be involved in the response to the external environment. The above results suggest that LoSorNINJA1 and LoSorNINJA2 might have undergone functional differentiation, and their functional sites and developmental stages might also be different. The present study provides basic data for in-depth analysis of the function of NINJAs in lily dormancy release.
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