Identification and Expression Analysis of OSCA Gene Family in Ginger (Zingiber officinale)
LONG Cha1,2,3, WANG Tao1,2, HUA He-Lan4, WU Yuan1,2, SU Shi-Xian1, WEI Jin-Jiang1,2, JIANG Su-Yan1, MOU Hai-Lin1,2, ZHU Hong-Jiang1,2, LYU Jin-Li5, XIA Ying-Shu6, LI Wei1,2,3,*
1 College of Agriculture, Guizhou University, Guiyang 550025, China; 2 Vegetable Research Institute, Guizhou University, Guiyang 550025, China; 3 Engineering Research Center for Protected Vegetable Crops in Higher Learning Institutions, Guiyang 550025, China; 4 Huaxi District Plant Protection Station, Guiyang, 550025, China; 5 Liupanshui Academy of Agricultural Sciences, Liupanshui 553000, China; 6 Guizhou Qiantangjiang Biotechnology Co., Ltd., Anshun, 561200 China
Abstract：Hyperosmolarity gate calcium-permeable channels (OSCA) is a mechanosensitive porous ion channel, which can regulate Ca2+ concentration and water transport rate in plant cells under osmotic stress. It plays an important role in hypertonic stress in plants. In order to understand the structural characteristics and expression characteristics of OSCA gene family in ginger, this study identified and analyzed the OSCA gene family in ginger (Zingiber officinale). The results showed that 38 members of OSCA gene family were identified from ginger genome, which were located on 14 chromosomes respectively. The prediction of subcellular localization shows that the members of OSCA family in ginger were mainly located in plasma membrane. The analysis of cis-acting elements of promoters showed that 38 ZoOSCAs contained various response elements to adversity and hormone, such as methyl jasmonate, abscisic acid and drought. The transcriptome of salt stress and fluorescence quantitative results of drought treatment showed that the expression levels of 18, 23 family members in leaves and rhizomes were up-regulated under salt stress, and 11 out of 14 family members were up-regulated under drought treatment, and the expression levels of ZoOSCAs in different tissues were different. This study provides basic data for revealing the function and mechanism of ZoOSCAs.
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