Progress in the Application of CRISPR/Cas9 Gene Editing Technology in Pest Gene Function and Pest Control Research
XIANG Yun-Ju1, ZHANG Wang-He1,*, XU Jin1,2,*, YE Hui3
1 Yunnan Key Laboratory of Forest Disaster Warning and Control, Southwest Forestry University, Kunming 650224, China; 2 Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming 650224, China; 3 School of Ecology and Environment, Yunnan University, Kunming 650091, China
Abstract：CRISPR/Cas9 technology, as the third generation of gene editing technology with simple design, convenient and quick operation, and low cost of use, has set off a technological revolution in the field of life sciences research. Currently, CRISPR/Cas9 has been successfully applied to gene editing in eukaryotic and prokaryotic organisms such as animals, plants and microorganisms. In recent years, CRISPR/Cas9 has also been used in the study of insects, and has shown great promise in the exploration of new technologies for pest control. This paper mainly provides an overview of the working principle of the CRISPR/Cas9 system and summarizes the progress of this technology in pest gene function and pest control. This review provides a reference for the use of CRISPR/Cas9 to carry out broader research in insect molecular biology and pest management strategies.
向云菊, 张旺和, 徐进, 叶辉. CRISPR/Cas9基因编辑技术在害虫基因功能及害虫防治研究中的应用进展[J]. 农业生物技术学报, 2024, 32(6): 1415-1431.
XIANG Yun-Ju, ZHANG Wang-He, XU Jin, YE Hui. Progress in the Application of CRISPR/Cas9 Gene Editing Technology in Pest Gene Function and Pest Control Research. 农业生物技术学报, 2024, 32(6): 1415-1431.
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