Abstract:To understand the global patent landscape and industrial development trends of agricultural animal gene editing technology, in present study, patent bibliometrics were used to analyze patent data in the gene editing technology field from 2015 to 2022. A systematic analysis was conducted, including major research institutions, research teams, research frontiers, and the overview of gene editing technology for different agricultural animal species. The results showed that gene editing technology patents had been on the rise globally over the past 8 years. China ranked first in the world in terms of the number of gene editing technology patents applied and published, but overall, the United States was leading the world in gene editing technology. Particularly in terms of core technology innovation, the quality of papers and patents, top research institutions and teams, transformation of knowledge achievements, and industrialization mechanisms, China still needs to improve further. In China, the number of gene editing technology patents related to agricultural animal breeding was relatively small. However, some related companies had already completed first-round financing at the level of 10 millions. This study provides a systematic analysis of the global industrialization trends of agricultural animal gene editing technology, and provides a reference for China to use gene editing technology to address major problems in agricultural animal breeding.
张萌萌, 蒋颖, 李宁. 全球基因编辑技术产业化发展分析及其在农业动物育种中的应用[J]. 农业生物技术学报, 2024, 32(3): 691-700.
ZHANG Meng-Meng, JIANG Ying, LI Ning. Analysis of the Global Gene Editing Industrialization and Its Application in Agricultural Animal Breeding. 农业生物技术学报, 2024, 32(3): 691-700.
[1] 范月蕾, 王慧媛, 王恒哲, 等. 2018. 国内外CRISPR/Cas9基因编辑专利技术发展分析[J]. 生命科学, 30: 1010-1018. (Fan Y L, Wang H Y, Wang H Z, et al.2018. Patent analysis on the development of domestic and foreign gene editing technologies[J]. Chinese Bulletin of Life Sciences, 30: 1010-1018.) [2] 宋巧枝, 方曙. 2008. 基于文献统计分析法的专利计量分析研究[J]. 现代情报,(02): 125-126;129. (Song Q Z, Fang S.2008. Research on patent quantitative analysis based on literature statistical analysis method[J]. Modern Information,(02): 125-126;129.) [3] 徐景, 杨光, 江美祺, 等. 2022. CRISPR/Cas9技术在畜禽育种中的研究进展[J]. 中国畜牧兽医, 49: 1374-1383. (Xu J, Yang G, Jiang M Q, et al.2022. Research advances on CRISPR/Cas9 technology in livestock and poultry breeding[J]. China Animal Husbandry & Veterinary Medicine, 49: 1374-1383.) [4] 许丽, 王玥, 姚驰远, 等. 2018. 基因编辑技术发展态势分析与建议[J]. 中国生物工程杂志, 38: 113-122. (Xu L, Wang Y, Yao C Y, et al.2018. Trends and development bottleneck analysis of gene editing technology[J]. China Biotechnology, 38: 113-122.) [5] 邹婉侬, 宋敏. 2020. 基于专利数据的植物基因编辑技术发展动态与竞争态势分析[J]. 农业生物技术学报, 28(6): 1060-1072. (Zou W N, Song M.2020. Analysis on the development trend and competitive situation of plant gene editing technology based on patent information[J]. Journal of Agricultural Biotechnology, 28(6): 1060-1072.) [6] Becker R.2015. US government approves transgenic chicken[J]. Nature, DOI: 10.1038/nature.2015.18985. [7] Burkard C, Lillico S G, Reid E, et al.2017. Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function[J]. PLOS Pathogens, 13: e1006206. [8] Cermak T, Doyle E L, Christian M, et al.2011. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting[J]. Nucleic Acids Research, 39(12): e82. [9] Duan Z, Liang Y, Sun J, et al.2024. An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plants[J]. The Innovation, DOI: 10.1016/j.xinn. 2024.100564. [10] FDA.2020. FDA approves first-of-its-kind intentional genomic alteration in line of domestic pigs for both human food, potential therapeutic uses[N]. https://www.fda.gov/news-events/press-announcements/fda-approves-first-its-kind-intentional-genomic-alteration-line-domestic-pigs-both-human-food. [11] Garneau J E, Dupuis M-È, Villion M, et al.2010. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA[J]. Nature, 468: 67-71. [12] Geurts A M, Cost G J, Freyvert Y, et al.2009. Knockout rats via embryo microinjection of zinc-finger nucleases[J]. Science, 325: 433. [13] Heavey S.2009. US approves first drug from dna-altered animals[N]. Science News, Reuters, February 6, 2009. https://www.reuters.com/article/idUSTRE5154OE. [14] Ishino Y, Shinagawa H, Makino K, et al.1987. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product[J]. Journal of Bacteriology, 169: 5429-5433. [15] Jinek M, Chylinski K, Fonfara I, et al.2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity[J]. Science, 337: 816-821. [16] Kershanskaya O I, Yessenbaeva G L, Nelidova D S, et al.2022. CRISPR/Cas genome editing perspectives for barley breeding[J]. Physiologia Plantarum, 174: e13686. [17] Nature.2009. Transgenic drug gets green light from the United States[J]. Nature, 457: 775. [18] Nature Biotechnology.2014. Rabbit milk Ruconest for hereditary angioedema[J]. Nature Biotechnology, 32: 849. [19] Nature Biotechnology.2022. Japan embraces CRISPR-edited fish[J]. Nature Biotechnology, 40: 10. [20] Wang J Y, Doudna J A2023. CRISPR technology: A decade of genome editing is only the beginning[J]. Science, 379: eadd8643. [21] Whitworth K M, Rowland R R, Petrovan V, et al.2019. Resistance to coronavirus infection in amino peptidase N-deficient pigs[J]. Transgenic Research, 28: 21-32. [22] Xu K, Zhou Y, Mu Y, et al.2020. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance[J]. Elife, 9: e57132.