Cloning and Functional Analysis of CtWRI1 Gene from Safflower (Carthamus tinctorius)
LI Jia-Qi1, REN Ying-Ying2, LIU Sha1, LIU Hong-Ye1, TIAN Yin-Shuai1,*
1 School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China; 2 College of Life Sciences, Sichuan University, Chengdu 610065, China
Abstract：Safflower (Carthamus tinctorius) is an economic crop with the medicinal and oil values, and analyzing the molecular mechanism of safflower seed oil biosynthesis will be benefiting for the genetic breeding of safflower. WRINKLED1 (WRI1) gene plays an important regulatory role in plant lipid biosynthesis. In order to analyze the molecular mechanism of oil accumulation in safflower seeds, the CDS sequence (GenBank No. OP785157) of safflower CtWRI1 gene was cloned from 'Yuhonghua No. 3' combined with the early transcriptomic data and homologous sequence method, and its bioinformatics and expression patterns were analyzed. The function of CtWRI1 gene was identified through tobacco (Nicotiana tabacum) genetic transformation method. The results showed that the total CDS length of CtWRI1 was 1 128 bp, encoding 375 amino acids. The CtWRI1 protein was predicted containing 2 conserved AP2/ERF domains. CtWRI1 protein sequence was closely related to sunflower (Helianthus annuus) and lettuce (Lactuca sativa) by the evolutionary tree analysis. The expression level of CtWRI1 was high during seed development and reached the highest level in 10-days embryo. The analysis results of CtWRI1 transgenic tobacco showed that CtWRI1 could induce the expression of genes involved in fatty acid biosynthesis, and the oil content of transgenic tobacco seeds was significantly increased. This study lays the foundation for further study of the molecular mechanism of oil biosynthesis in safflower seeds.
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